In summary, we have adapted the CTAB method to the extraction of LMW RNA from polysaccharide-rich plant tissues. LMW RNA obtained by the method presented is pure and intact, and can be loaded in large amount in denaturing PAGE. Thus, it can be used for siRNA detection and miRNA expression analysis by northern blotting, and to produce small RNA libraries from plant organs, such as fruits and seeds, in which the role of these small RNA molecules has been, thus far, little explored.

A cetyltrimethylammonium bromide-based method to extract low-molecular-weight RNA from polysaccharide-rich plant tissues

CARRA, Andrea Massimo;GAMBINO, Giorgio;SCHUBERT, Andrea
2006

Abstract

In summary, we have adapted the CTAB method to the extraction of LMW RNA from polysaccharide-rich plant tissues. LMW RNA obtained by the method presented is pure and intact, and can be loaded in large amount in denaturing PAGE. Thus, it can be used for siRNA detection and miRNA expression analysis by northern blotting, and to produce small RNA libraries from plant organs, such as fruits and seeds, in which the role of these small RNA molecules has been, thus far, little explored.
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http://www.elsevier.com/wps/find/journaldescription.cws_home/622781/description#description
RNA extraction; microRNA
CARRA A; GAMBINO G; SCHUBERT A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/9851
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