The labeling of proteins employing succinimido 4-pentynoate or pyrylium derivatives of mono- and dinuclear organometallic complexes is now a well-established procedure. We have covalently linked a 5d trimetallic cluster fragment, namely ("OS3(CO)(10)", to a model amine the. benzylamine), to model amino acids (i.e. beta-alanine and N alpha-protected lysines), and finally to bovine serum albumin (BSA). The complete reaction sequence, starting from Os-3(CO)(10)(mu(3)-eta(2)-succinimido 4-pentynoate) as the Bolton-Hunter-like reagent, was verified at the molecular level by spectroscopic techniques. The average coupling ratio (CR) between the Os-3 marker and BSA is fairly high, i.e. around 20. This result highlights the potential use of the Os-3-labeling procedure for improving the X-ray structural determination and transmission electron microscopy (TEM) definition of proteins.
Labeling of Proteins by a Triosmium Carbonyl Cluster via a Bolton-Hunter-like Procedure
VINCENTI, Marco;
1996-01-01
Abstract
The labeling of proteins employing succinimido 4-pentynoate or pyrylium derivatives of mono- and dinuclear organometallic complexes is now a well-established procedure. We have covalently linked a 5d trimetallic cluster fragment, namely ("OS3(CO)(10)", to a model amine the. benzylamine), to model amino acids (i.e. beta-alanine and N alpha-protected lysines), and finally to bovine serum albumin (BSA). The complete reaction sequence, starting from Os-3(CO)(10)(mu(3)-eta(2)-succinimido 4-pentynoate) as the Bolton-Hunter-like reagent, was verified at the molecular level by spectroscopic techniques. The average coupling ratio (CR) between the Os-3 marker and BSA is fairly high, i.e. around 20. This result highlights the potential use of the Os-3-labeling procedure for improving the X-ray structural determination and transmission electron microscopy (TEM) definition of proteins.
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