The labeling of proteins employing succinimido 4-pentynoate or pyrylium derivatives of mono- and dinuclear organometallic complexes is now a well-established procedure. We have covalently linked a 5d trimetallic cluster fragment, namely ("OS3(CO)(10)", to a model amine the. benzylamine), to model amino acids (i.e. beta-alanine and N alpha-protected lysines), and finally to bovine serum albumin (BSA). The complete reaction sequence, starting from Os-3(CO)(10)(mu(3)-eta(2)-succinimido 4-pentynoate) as the Bolton-Hunter-like reagent, was verified at the molecular level by spectroscopic techniques. The average coupling ratio (CR) between the Os-3 marker and BSA is fairly high, i.e. around 20. This result highlights the potential use of the Os-3-labeling procedure for improving the X-ray structural determination and transmission electron microscopy (TEM) definition of proteins.

Labeling of Proteins by a Triosmium Carbonyl Cluster via a Bolton-Hunter-like Procedure

VINCENTI, Marco;
1996-01-01

Abstract

The labeling of proteins employing succinimido 4-pentynoate or pyrylium derivatives of mono- and dinuclear organometallic complexes is now a well-established procedure. We have covalently linked a 5d trimetallic cluster fragment, namely ("OS3(CO)(10)", to a model amine the. benzylamine), to model amino acids (i.e. beta-alanine and N alpha-protected lysines), and finally to bovine serum albumin (BSA). The complete reaction sequence, starting from Os-3(CO)(10)(mu(3)-eta(2)-succinimido 4-pentynoate) as the Bolton-Hunter-like reagent, was verified at the molecular level by spectroscopic techniques. The average coupling ratio (CR) between the Os-3 marker and BSA is fairly high, i.e. around 20. This result highlights the potential use of the Os-3-labeling procedure for improving the X-ray structural determination and transmission electron microscopy (TEM) definition of proteins.
1996
15
3037
3041
http://10.0.3.253/om960088i
http://www.researchgate.net/publication/224842567_Labeling_of_proteins_by_a_triosmium_carbonyl_cluster_via_a_Bolton-Hunter-like_procedure
D. Osella; M. Ravera; M. Vincenti; M. Salmain; G. Jaouen
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/109338
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