Previously PFGE-typed Listeria monocytogenes isolates (N=95) were tested by Repetitive element sequence-based PCRs. A combined rep-PCR typing approach showed 95% repeatability; 0.98 discriminatory power; 95.5% sensitivity; 75% specificity; 91.2% predictive positive value, and 85.7% predictive negative value. Hence, rep-PCR represent an efficient and rapid subtyping method for L. monocytogenes.
Diagnostic performance of rep-PCR as a rapid subtyping method for Listeria monocytogenes
NUCERA, Daniele Michele;LOMONACO, Sara;COSTA, ANNALISA;MORRA, Patrizia;GRASSI, Maria Ausilia
2013-01-01
Abstract
Previously PFGE-typed Listeria monocytogenes isolates (N=95) were tested by Repetitive element sequence-based PCRs. A combined rep-PCR typing approach showed 95% repeatability; 0.98 discriminatory power; 95.5% sensitivity; 75% specificity; 91.2% predictive positive value, and 85.7% predictive negative value. Hence, rep-PCR represent an efficient and rapid subtyping method for L. monocytogenes.
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