Highly bright and photostable cyanine dye-doped silica nanoparticles, IRIS Dots, are developed, which can efficiently label human mesenchymal stem cells (hMSCs). The application procedure used to label hMSCs is fast (2 h), the concentration of IRIS Dots for efficient labeling is low (20 μg mL(-1) ), and the labeled cells can be visualized by flow cytometry, confocal microscopy, and transmission electron microscopy. Labeled hMSCs are unaffected in their viability and proliferation, as well as stemness surface marker expression and differentiation capability into osteocytes. Moreover, this is the first report that shows nonfunctionalized IRIS Dots can discriminate between live and early-stage apoptotic stem cells (both mesenchymal and embryonic) through a distinct external cell surface distribution. On the basis of biocompatibility, efficient labeling, and apoptotic discrimination potential, it is suggested that IRIS Dots can serve as a promising stem cell tracking agent.

Fluorescent Silica Nanoparticles Improve Optical Imaging of Stem Cells Allowing Direct Discrimination between Live and Early-Stage Apoptotic Cells

ACCOMASSO, LISA;CIBRARIO ROCCHIETTI, ELISA;RAIMONDO, Stefania;CATALANO, FEDERICO;ALBERTO, Gabriele;MINIERI, VALENTINA;TURINETTO, VALENTINA;ORLANDO, Luca;SAVIOZZI, Silvia;CAPUTO, Giuseppe;GEUNA, Stefano;MARTRA, Gianmario;GIACHINO, Claudia
2012-01-01

Abstract

Highly bright and photostable cyanine dye-doped silica nanoparticles, IRIS Dots, are developed, which can efficiently label human mesenchymal stem cells (hMSCs). The application procedure used to label hMSCs is fast (2 h), the concentration of IRIS Dots for efficient labeling is low (20 μg mL(-1) ), and the labeled cells can be visualized by flow cytometry, confocal microscopy, and transmission electron microscopy. Labeled hMSCs are unaffected in their viability and proliferation, as well as stemness surface marker expression and differentiation capability into osteocytes. Moreover, this is the first report that shows nonfunctionalized IRIS Dots can discriminate between live and early-stage apoptotic stem cells (both mesenchymal and embryonic) through a distinct external cell surface distribution. On the basis of biocompatibility, efficient labeling, and apoptotic discrimination potential, it is suggested that IRIS Dots can serve as a promising stem cell tracking agent.
2012
8
20
3192
3200
Accomasso L; Cibrario Rocchietti E; Raimondo S; Catalano F; Alberto G; Giannitti A; Minieri V; Turinetto V; Orlando L; Saviozzi S; Caputo G; Geuna S; ...espandi
File in questo prodotto:
File Dimensione Formato  
Accomasso et al_definitivo.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 1.53 MB
Formato Adobe PDF
1.53 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Accomasso et al_Supporting Information.pdf

Accesso riservato

Tipo di file: MATERIALE NON BIBLIOGRAFICO
Dimensione 233.38 kB
Formato Adobe PDF
233.38 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/121775
Citazioni
  • ???jsp.display-item.citation.pmc??? 15
  • Scopus 34
  • ???jsp.display-item.citation.isi??? 34
social impact