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EnglishBarley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
| Titolo: | A physical, genetic and functional sequence assembly of the barley genome. | |
| Autori Riconosciuti: | ||
| Autori: | Mayer KF; Waugh R; Langridge P; Close TJ; Wise RP; Graner A; Matsumoto T; Sato K; Schulman A; Muehlbauer GJ; Stein N; Ariyadasa R; Schulte D; Poursarebani N; Zhou R; Steuernagel B; Mascher M; Scholz U; Shi B; Langridge P; Madishetty K; Svensson JT; Bhat P; Moscou M; Resnik J; Close TJ; Muehlbauer GJ; Hedley P; Liu H; Morris J; Waugh R; Frenkel Z; Korol A; Bergès H; Graner A; Stein N; Steuernagel B; Scholz U; Taudien S; Felder M; Groth M; Platzer M; Stein N; Steuernagel B; Scholz U; Himmelbach A; Taudien S; Felder M; Platzer M; Lonardi S; Duma D; Alpert M; Cordero F; Beccuti M; Ciardo G; Ma Y; Wanamaker S; Close TJ; Stein N; Cattonaro F; Vendramin V; Scalabrin S; Radovic S; Wing R; Schulte D; Steuernagel B; Morgante M; Stein N; Waugh R; Nussbaumer T; Gundlach H; Martis M; Ariyadasa R; Poursarebani N; Steuernagel B; Scholz U; Wise RP; Poland J; Stein N; Mayer KF; Spannagl M; Pfeifer M; Gundlach H; Mayer KF; Gundlach H; Moisy C; Tanskanen J; Scalabrin S; Zuccolo A; Vendramin V; Morgante M; Mayer KF; Schulman A; Pfeifer M; Spannagl M; Hedley P; Morris J; Russell J; Druka A; Marshall D; Bayer M; Swarbreck D; Sampath D; Ayling S; Febrer M; Caccamo M; Matsumoto T; Tanaka T; Sato K; Wise RP; Close TJ; Wannamaker S; Muehlbauer GJ; Stein N; Mayer KF; Waugh R; Steuernagel B; Schmutzer T; Mascher M; Scholz U; Taudien S; Platzer M; Sato K; Marshall D; Bayer M; Waugh R; Stein N; Mayer KF; Waugh R; Brown JW; Schulman A; Langridge P; Platzer M; Fincher GB; Muehlbauer GJ; Sato K; Close TJ; Wise RP; Stein N. | |
| Data di pubblicazione: | 2012 | |
| Abstract: | Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement. | |
| Volume: | 491 | |
| Pagina iniziale: | 711 | |
| Pagina finale: | 716 | |
| Digital Object Identifier (DOI): | 10.1038/nature11543 | |
| Parole Chiave: | Genomics; Plant sciences; Genetics | |
| Rivista: | NATURE | |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| A physical, genetic and functional sequence assembly of the barley genome.pdf | POSTPRINT (VERSIONE FINALE DELL’AUTORE) | Open Access Visualizza/Apri |
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