The genus Mentha is of particular economic importance. The development of new methods for the characterisation of Mentha species is crucial for their unequivocal identification. Amplification of the non-transcribed spacer (NTS) of the 5S-rRNA gene was used to characterise some Mentha species, which revealed a high-specific variability. Cloning and sequencing of all amplified NTS fragments enabled the discrimination among almost all species. In silico and experimental analyses identified specific restriction sites on the amplified 5S-NTS regions, facilitating the rapid and unambiguous discrimination of all the different species by polymerase chain reaction–restriction fragment length polymorphism. A direct comparison between essential oil composition and DNA fingerprinting confirmed a relationship between chemical and molecular data.
Molecular fingerprinting of some Mentha species by sequencing and RFLP analysis of the 5S rRNA non-transcribed spacer (NTS) region
CAPUZZO, ANDREA;MAFFEI, Massimo Emilio
2014-01-01
Abstract
The genus Mentha is of particular economic importance. The development of new methods for the characterisation of Mentha species is crucial for their unequivocal identification. Amplification of the non-transcribed spacer (NTS) of the 5S-rRNA gene was used to characterise some Mentha species, which revealed a high-specific variability. Cloning and sequencing of all amplified NTS fragments enabled the discrimination among almost all species. In silico and experimental analyses identified specific restriction sites on the amplified 5S-NTS regions, facilitating the rapid and unambiguous discrimination of all the different species by polymerase chain reaction–restriction fragment length polymorphism. A direct comparison between essential oil composition and DNA fingerprinting confirmed a relationship between chemical and molecular data.File | Dimensione | Formato | |
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Molecular_fingerprinting_of_some_4aperto.pdf
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2014 Plan biosyst Mentha Species DNA.pdf
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