Intracellular calcium signals activated by growth factors in endothelial cells during angiogenesis regulate cytosolic and nuclear events involved in survival, proliferation and motility. Among the intracellular messengers released upon proangiogenic stimulation, arachidonic acid (AA) and its metabolites play a key role, and their effects are strictly related to calcium homeostasis. In human breast tumor-derived endothelial cells (B-TECs) AA stimulates proliferation and tubulogenesis in a calcium-dependent way. Here, to characterize the proteins whose expression is regulated by AA-induced calcium entry, we used a proteomic approach (two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry, 2-DE and MALDI-MS) and we compared the proteomes of B-TECs stimulated with AA in presence or in absence of calcium entry (with addition to the culture medium of the calcium chelator EGTA, which completely prevents calcium fluxes throughout the plasma membrane). We found that six proteins increased their levels of expression, all higher when AA-induced calcium entry was abolished. These proteins have been identified by mass spectrometry and database search, and their potential roles in AA-stimulated pathway and in angiogenesis are discussed.

Arachidonic acid and calcium signals in human breast tumor-derived endothelial cells: a proteomic study

ANTONIOTTI, Susanna;FATTORI, Paolo;PESSIONE, Enrica;MUNARON, Luca Maria
2009

Abstract

Intracellular calcium signals activated by growth factors in endothelial cells during angiogenesis regulate cytosolic and nuclear events involved in survival, proliferation and motility. Among the intracellular messengers released upon proangiogenic stimulation, arachidonic acid (AA) and its metabolites play a key role, and their effects are strictly related to calcium homeostasis. In human breast tumor-derived endothelial cells (B-TECs) AA stimulates proliferation and tubulogenesis in a calcium-dependent way. Here, to characterize the proteins whose expression is regulated by AA-induced calcium entry, we used a proteomic approach (two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry, 2-DE and MALDI-MS) and we compared the proteomes of B-TECs stimulated with AA in presence or in absence of calcium entry (with addition to the culture medium of the calcium chelator EGTA, which completely prevents calcium fluxes throughout the plasma membrane). We found that six proteins increased their levels of expression, all higher when AA-induced calcium entry was abolished. These proteins have been identified by mass spectrometry and database search, and their potential roles in AA-stimulated pathway and in angiogenesis are discussed.
29
5
257
265
Susanna Antoniotti; Paolo Fattori; Cristiana Tomatis; Enrica Pessione; Luca Munaron
File in questo prodotto:
File Dimensione Formato  
Journal of Receptors and Signal Transduction 2009 Antoniotti.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 874.02 kB
Formato Adobe PDF
874.02 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/128504
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 4
social impact