Conjugated linoleic acid (CLA) has beneficial effects against breast carcinogenesis. In its antiproliferative action we hypothesized a cross-talk between PPARbeta and E-cadherin/ß-catenin systems, important in cellular adhesion and signal transduction. ß-catenin system is regulated by ERK and PI3K/Akt cascades, crucial in growth signal transmission. We tested CLA interference with these pathways in ERα+ (MCF-7) and ERα- (MDA-MB-231) breast cancer cells. We used immunoblotting, immunofluorescence, immunoprecipitation, zymography, Boyden chamber techniques. CLA is more effective in MCF-7 cells: it transactivates PPARbeta and induces E-cadherin/ß-catenin complex formation at plasmamembrane. In MDA-MB-231 cells upregulates PPARα and induces apoptosis. In both cell lines inhibits ERK and PI3K/Akt signalling and reduces cell invasiveness. CLA action involves signalling pathways recently considered as new targets for anti-cancer strategies.

A Cla response of Human Breast cancer cells: mechanisms involved / Bocca C; Bozzo F; Cannito S; Colombatto S; Miglietta A.. - In: THE AMERICAN JOURNAL OF PATHOLOGY. - ISSN 0002-9440. - STAMPA. - 173(2008), pp. S9--. ((Intervento presentato al convegno XXIX National Congress Italian Society of Pathology tenutosi a Rende (CS) nel 10-13 Settembre 2008.

A Cla response of Human Breast cancer cells: mechanisms involved

BOCCA, Claudia;BOZZO, FRANCESCA;CANNITO, STEFANIA;COLOMBATTO, Sebastiano;MIGLIETTA, Antonella
2008

Abstract

Conjugated linoleic acid (CLA) has beneficial effects against breast carcinogenesis. In its antiproliferative action we hypothesized a cross-talk between PPARbeta and E-cadherin/ß-catenin systems, important in cellular adhesion and signal transduction. ß-catenin system is regulated by ERK and PI3K/Akt cascades, crucial in growth signal transmission. We tested CLA interference with these pathways in ERα+ (MCF-7) and ERα- (MDA-MB-231) breast cancer cells. We used immunoblotting, immunofluorescence, immunoprecipitation, zymography, Boyden chamber techniques. CLA is more effective in MCF-7 cells: it transactivates PPARbeta and induces E-cadherin/ß-catenin complex formation at plasmamembrane. In MDA-MB-231 cells upregulates PPARα and induces apoptosis. In both cell lines inhibits ERK and PI3K/Akt signalling and reduces cell invasiveness. CLA action involves signalling pathways recently considered as new targets for anti-cancer strategies.
XXIX National Congress Italian Society of Pathology
Rende (CS)
10-13 Settembre 2008
173
S9
-
Bocca C; Bozzo F; Cannito S; Colombatto S; Miglietta A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/131700
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