The dynamics of plant volatile (PV) emission, and the relationship between damaged area and biosynthesis of bioactive molecules in plant-insect interactions, remain open questions. Direct Contact-Sorptive Tape Extraction (DC-STE) is a sorption sampling technique employing non adhesive polydimethylsiloxane tapes, which are placed in direct contact with a biologically-active surface. DC-STE coupled to Gas Chromatography - Mass Spectrometry (GC-MS) is a non-destructive, high concentration-capacity sampling technique able to detect and allow identification of PVs involved in plant responses to biotic and abiotic stresses. Here we investigated the leaf topographical dynamics of herbivory-induced PV (HIPV) produced by Phaseolus lunatus L. (lima bean) in response to herbivory by larvae of the Mediterranean climbing cutworm (Spodoptera littoralis Boisd.) and mechanical wounding by DC-STE-GC-MS.

Direct Contact - Sorptive Tape Extraction coupled with Gas Chromatography - Mass Spectrometry to reveal volatile topographical dynamics of lima bean (Phaseolus lunatus L.) upon herbivory by Spodoptera littoralis Boisd

BOGGIA, LORENZO;SGORBINI, Barbara;BERTEA, CINZIA MARGHERITA;CAGLIERO, Cecilia Lucia;BICCHI, Carlo;MAFFEI, Massimo Emilio;RUBIOLO, Patrizia
2015

Abstract

The dynamics of plant volatile (PV) emission, and the relationship between damaged area and biosynthesis of bioactive molecules in plant-insect interactions, remain open questions. Direct Contact-Sorptive Tape Extraction (DC-STE) is a sorption sampling technique employing non adhesive polydimethylsiloxane tapes, which are placed in direct contact with a biologically-active surface. DC-STE coupled to Gas Chromatography - Mass Spectrometry (GC-MS) is a non-destructive, high concentration-capacity sampling technique able to detect and allow identification of PVs involved in plant responses to biotic and abiotic stresses. Here we investigated the leaf topographical dynamics of herbivory-induced PV (HIPV) produced by Phaseolus lunatus L. (lima bean) in response to herbivory by larvae of the Mediterranean climbing cutworm (Spodoptera littoralis Boisd.) and mechanical wounding by DC-STE-GC-MS.
BMC PLANT BIOLOGY
15
1:102
1
13
http://www.biomedcentral.com/1471-2229/15/102
Direct Contact-Sorptive Tape Extraction (DC-STE); Gas Chromatography coupled with Mass Spectrometry (GC-MS); Herbivory-induced plant volatile (HIPV); Phaseolus lunatus L.; Spodoptera littoralis Boisd.; Plant-insect interactions; Herbivory; Green leaf volatiles (GLVs); Monoterpenoids; Sesquiterpenoids
Boggia, Lorenzo; Sgorbini, Barbara; Bertea, Cinzia M; Cagliero, Cecilia; Bicchi, Carlo; Maffei, Massimo E; Rubiolo, Patrizia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1520225
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