Mutations of the BCR-ABL1 fusion gene represent a well established cause of resistance to tyrosine kinase inhibitors. Among the different mutations identified T315I is of particular concern since it is not effectively targeted by the majority of Tyrosine Kinase Inhibitors so far available. We developed a novel assay based on peptide nucleic acid (PNA) technology coupled to immunofluorescence microscopy (PNA-FISH) for the specific detection at a single cell level of BCR-ABL (T315I) mutation thus improving both, diagnostic resolution and the study of clonal prevalence. Furthermore we developed an additional method based on PNA directed PCR-clamping for the fast and easy detection of the mutation.

Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH

ROSSO, VALENTINA;BRACCO, Enrico
Co-first
;
CARTURAN, Sonia;SIGNORINO, Elisabetta;PETITI, JESSICA;CALABRESE, CHIARA;NICOLI, Paolo;DE GOBBI, Marco;GAIDANO, Valentina;GALLO, Daniela;ULISCIANI, STEFANO;FAVA, Carmen;SAGLIO, Giuseppe;CILLONI, Daniela
Last
2015-01-01

Abstract

Mutations of the BCR-ABL1 fusion gene represent a well established cause of resistance to tyrosine kinase inhibitors. Among the different mutations identified T315I is of particular concern since it is not effectively targeted by the majority of Tyrosine Kinase Inhibitors so far available. We developed a novel assay based on peptide nucleic acid (PNA) technology coupled to immunofluorescence microscopy (PNA-FISH) for the specific detection at a single cell level of BCR-ABL (T315I) mutation thus improving both, diagnostic resolution and the study of clonal prevalence. Furthermore we developed an additional method based on PNA directed PCR-clamping for the fast and easy detection of the mutation.
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BCR-ABL1; Chronic Myeloid Leukemia; PNA; T315I mutation
Rosso, Valentina; Bracco, Enrico; Pedrola, Roberto; Carturan, Sonia; Signorino, Elisabetta; Petiti, Jessica; Calabrese, Chiara; Nicoli, Paolo; De Gobbi, Marco; Gaidano, Valentina; Gallo, Daniela; Ulisciani, Stefano; Fava, Carmen; Rege-Cambrin, Giovanna; Frassoni, Francesco; Saglio, Giuseppe; Cilloni, Daniela
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1522337
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