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EnglishPlant NADPH oxidases, known as respiratory burst oxidase homologs (RBOH), belong to a multigenic family that plays an important role in the regulation of plant development and responses to biotic and abiotic stresses. In this study, we monitored the expression profiles of five Rboh genes (MtRbohA, MtRbohB, MtRbohE, MtRbohG, MtRbohF) in the roots of the model species Medicago truncatula upon colonization by arbuscular mycorrhizal fungi. A complementary cellular and molecular approach was used to monitor changes in mRNA abundance and localize transcripts in different cell types from mycorrhizal roots. Rboh transcript levels did not drastically change in total RNA extractions from whole mycorrhizal and nonmycorrhizal roots. Nevertheless, the analysis of laser microdissected cells and Agrobacterium rhizogenestransformed rhizogenestransformed roots expressing a GUS transcriptional fusion construct highlighted the MtRbohE expression in arbuscule- containing cells. Furthermore, the down regulation of MtRbohE by an RNAi approach generated an altered colonization pattern in the root cortex, when compared to control roots, with fewer arbuscules and multiple penetration attempts. Altogether our data indicate a transient upregulation of MtRbohE expression in cortical cells colonized by arbuscules and suggest a role for MtRbohE in arbuscule accommodation within cortical cells.
| Titolo: | The Medicago truncatulaMtRbohE gene is activated in arbusculated cells and is involved in root cortex colonization |
| Autori Riconosciuti: | |
| Autori: | Belmondo, Simone; Calcagno, Cristina; Genre, Andrea; Puppo, Alain; Pauly, Nicolas; Lanfranco, Luisa |
| Data di pubblicazione: | 2016 |
| Abstract: | Plant NADPH oxidases, known as respiratory burst oxidase homologs (RBOH), belong to a multigenic family that plays an important role in the regulation of plant development and responses to biotic and abiotic stresses. In this study, we monitored the expression profiles of five Rboh genes (MtRbohA, MtRbohB, MtRbohE, MtRbohG, MtRbohF) in the roots of the model species Medicago truncatula upon colonization by arbuscular mycorrhizal fungi. A complementary cellular and molecular approach was used to monitor changes in mRNA abundance and localize transcripts in different cell types from mycorrhizal roots. Rboh transcript levels did not drastically change in total RNA extractions from whole mycorrhizal and nonmycorrhizal roots. Nevertheless, the analysis of laser microdissected cells and Agrobacterium rhizogenestransformed rhizogenestransformed roots expressing a GUS transcriptional fusion construct highlighted the MtRbohE expression in arbuscule- containing cells. Furthermore, the down regulation of MtRbohE by an RNAi approach generated an altered colonization pattern in the root cortex, when compared to control roots, with fewer arbuscules and multiple penetration attempts. Altogether our data indicate a transient upregulation of MtRbohE expression in cortical cells colonized by arbuscules and suggest a role for MtRbohE in arbuscule accommodation within cortical cells. |
| Volume: | 243 |
| Fascicolo: | 1 |
| Pagina iniziale: | 251 |
| Pagina finale: | 262 |
| Digital Object Identifier (DOI): | 10.1007/s00425-015-2407-0 |
| URL: | link.springer.de/link/service/journals/00425/index.htm |
| Parole Chiave: | Arbuscular mycorrhizal symbiosis; Gene expression; Medicago; NADPH oxidase; Reactive oxygen species; Respiratory burst oxidase homolog; Plant Science; Genetics |
| Rivista: | PLANTA |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Belmondo et al 2015 Planta.pdf | PDF EDITORIALE | Utenti riconosciuti Richiedi una copia | ||
| Post print Belmondo et al 2016 1.pdf | Belmondo et al post print | POSTPRINT (VERSIONE FINALE DELL’AUTORE) | Open Access Visualizza/Apri |
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