Despite the ban imposed by EU, the triphenylmethane dye malachite green (MG) is still illegally used in aquaculture to treat fungal infestations and parasitic diseases. Previous in vitro studies indicate that in the trout MG could inhibit a number of liver drug metabolizing enzymes (DME) and suggest that it may be a CYP1A substrate (1). It is believed that the oxidative metabolism of MG results in the formation of a number of reactive species which may participate in the MG-mediated oxidative stress (OS) (2). This study was designed to characterize the effects of a short MG exposure on DME and OS in the rainbow trout and to study their modulation by the pretreatment with b-naphthoflavone (b-NAF), a model cytochrome P450 (CYP) 1A inducer.

Effects of malachite green exposure on liver drug metabolizing enzymes and oxidative stress in untreated versus b naphthoflavone pre-treated rainbow trouts (Oncorhyncus mykiss)

NEBBIA, Carlo;GASCO, Laura;GIROLAMI, Flavia;BADINO, Paola;ZOCCARATO, Ivo
2015

Abstract

Despite the ban imposed by EU, the triphenylmethane dye malachite green (MG) is still illegally used in aquaculture to treat fungal infestations and parasitic diseases. Previous in vitro studies indicate that in the trout MG could inhibit a number of liver drug metabolizing enzymes (DME) and suggest that it may be a CYP1A substrate (1). It is believed that the oxidative metabolism of MG results in the formation of a number of reactive species which may participate in the MG-mediated oxidative stress (OS) (2). This study was designed to characterize the effects of a short MG exposure on DME and OS in the rainbow trout and to study their modulation by the pretreatment with b-naphthoflavone (b-NAF), a model cytochrome P450 (CYP) 1A inducer.
13th International Congress of the European Association for Veterinary Pharmacology and Toxicology (EAVPT 2015),
Nantes, France
19–22 July 2015
Volume 38, Issue Supplement S1, 11.2
144
145
http://onlinelibrary.wiley.com/doi/10.1111/jvp.12247/abstract
triphenylmethane dye malachite green (MG), rainbow trout,
Nebbia Carlo, Giuliano Albo Alessandra, Gasco Laura, Girolami Flavia, Badino Paola, Zoccarato Ivo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1543196
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