A fluorescent immunochromatographic strip test (ICST) based on the use of Quantum Dots (QD) was developed and applied to detect fumonisins in maize samples. A limit of detection for fumonisin B1 of 2.8 mug/L was achieved, with an analytical working range of 3–350 mug/L, corresponding to 30–3500 mug/kg in maize flour samples, according with the extraction procedure. The time required to perform the analysis was 22 min, including sample preparation. Recovery values in the range from 91.4% to 105.4% with coefficients of variation not exceeding 5% were obtained for fortified and naturally contaminated maize flour samples. To evaluate the possible improvements due to the use of QD for ICST technology, we performed a direct comparison of the proposed QD-ICST to a gold nanoparticles- and a chemiluminescent-ICST previously developed for fumonisins detection, in which the same immunoreagents were employed

A fluorescent immunochromatographic strip test using quantum dots for fumonisins detection

DI NARDO, FABIO;ANFOSSI, Laura;GIOVANNOLI, Cristina;PASSINI, CINZIA;BAGGIANI, Claudio
2016-01-01

Abstract

A fluorescent immunochromatographic strip test (ICST) based on the use of Quantum Dots (QD) was developed and applied to detect fumonisins in maize samples. A limit of detection for fumonisin B1 of 2.8 mug/L was achieved, with an analytical working range of 3–350 mug/L, corresponding to 30–3500 mug/kg in maize flour samples, according with the extraction procedure. The time required to perform the analysis was 22 min, including sample preparation. Recovery values in the range from 91.4% to 105.4% with coefficients of variation not exceeding 5% were obtained for fortified and naturally contaminated maize flour samples. To evaluate the possible improvements due to the use of QD for ICST technology, we performed a direct comparison of the proposed QD-ICST to a gold nanoparticles- and a chemiluminescent-ICST previously developed for fumonisins detection, in which the same immunoreagents were employed
2016
150
463
468
Di Nardo, F.; Anfossi, L.; Giovannoli, C.; Passini, C.; Goftman, V.V.; Goryacheva, I.Y.; Baggiani, C.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1546003
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