Ki67 is associated with tumor grading and prognosis in different subtypes of human non-Hodgkin lymphoma. We recently reported that flow cytometric detection of Ki67 is useful in discriminating between high and low grade canine lymphomas, but its reliability as prognostic marker is still unclear. Aim of the study was to assess the prognostic significance of Ki67 in dogs with high grade B-cell lymphoma (HGB-LSA).45 HGB-LSAs treated with Wisconsin-Madison protocol were included. Diagnosis was based on flow cytometric immunophenotyping and cytology (updated Kiel classification). Ki67-positive cells (Ki67%) were determined by flow cytometry on lymph node aspirates using FITC-conjugated monoclonal antibody (clone MIB-1). Following variables were investigated for association with overall survival (OS) and relapse free interval (RFI) using univariate, multivariate and survival (Kaplan-Meyer) analyses: Ki67% (≤20%, 20.1-40%, >40%), breed (purebred or crossbred), sex (male or female), age (< or ≥ 10yrs), stage (I-IV or V), substage (a or b), complete remission (CR, yes or no). On multivariate analyses Ki67% (P=0.036) and achievement of CR (P=0.009) were independent prognostic factors for OS, while none of the considered variables reached significance for RFI. Dogs with Ki67=20.1-40% presented significantly longer OS and RFI (median=593 and 406 days, respectively) than dogs with Ki67≤20% (median=177 and 200 days) and Ki67%>40 (median= 258 and 142 days). Ki67 is an independent predictor of OS in treated HGB-LSAs; intermediate values are associated with best prognosis. We suggest flow cytometric determination of Ki67 as a diagnostic and prognostic tool to improve clinical usefulness of cytology and immunophenotyping.
Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high grade B-cell lymphoma
POGGI, ALESSIA;MINISCALCO, Barbara;MORELLO, Emanuela Maria;GATTINO, FRANCESCA;Aresu L;RIONDATO, Fulvio
2014-01-01
Abstract
Ki67 is associated with tumor grading and prognosis in different subtypes of human non-Hodgkin lymphoma. We recently reported that flow cytometric detection of Ki67 is useful in discriminating between high and low grade canine lymphomas, but its reliability as prognostic marker is still unclear. Aim of the study was to assess the prognostic significance of Ki67 in dogs with high grade B-cell lymphoma (HGB-LSA).45 HGB-LSAs treated with Wisconsin-Madison protocol were included. Diagnosis was based on flow cytometric immunophenotyping and cytology (updated Kiel classification). Ki67-positive cells (Ki67%) were determined by flow cytometry on lymph node aspirates using FITC-conjugated monoclonal antibody (clone MIB-1). Following variables were investigated for association with overall survival (OS) and relapse free interval (RFI) using univariate, multivariate and survival (Kaplan-Meyer) analyses: Ki67% (≤20%, 20.1-40%, >40%), breed (purebred or crossbred), sex (male or female), age (< or ≥ 10yrs), stage (I-IV or V), substage (a or b), complete remission (CR, yes or no). On multivariate analyses Ki67% (P=0.036) and achievement of CR (P=0.009) were independent prognostic factors for OS, while none of the considered variables reached significance for RFI. Dogs with Ki67=20.1-40% presented significantly longer OS and RFI (median=593 and 406 days, respectively) than dogs with Ki67≤20% (median=177 and 200 days) and Ki67%>40 (median= 258 and 142 days). Ki67 is an independent predictor of OS in treated HGB-LSAs; intermediate values are associated with best prognosis. We suggest flow cytometric determination of Ki67 as a diagnostic and prognostic tool to improve clinical usefulness of cytology and immunophenotyping.
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