Hyperpolarization of the 13C magnetic resonance signal of L-[1-13C]Lactate has been obtained using the chemically based, cost-effective method named ParaHydrogen Induced Polarization by means of Side Arm Hydrogenation (PHIP-SAH). Two ester derivatives of lactate have been tested and the factors that determine the polarization level on the product have been investigated into details. The metabolic conversion of hyperpolarized L-[1-13C]Lactate into Pyruvate has been observed in vitro using lactate dehydrogenase (LDH) and in a cells lysate. From the acquisition of a series of 13C-NMR spectra, the metabolic build-up of the [1-13C]Pyruvate signal has been observed. These studies demonstrate that, even if the experimental set-up used for these PHIP-SAH hyperpolarization studies is still far from optimal, the attained polarization level is already sufficient to carry out in vitro metabolic studies.

13C MR Hyperpolarization of Lactate by Using ParaHydrogen and Metabolic Transformation in Vitro

CAVALLARI, ELEONORA;CARRERA, CARLA;AIME, Silvio;REINERI, Francesca
Last
2017

Abstract

Hyperpolarization of the 13C magnetic resonance signal of L-[1-13C]Lactate has been obtained using the chemically based, cost-effective method named ParaHydrogen Induced Polarization by means of Side Arm Hydrogenation (PHIP-SAH). Two ester derivatives of lactate have been tested and the factors that determine the polarization level on the product have been investigated into details. The metabolic conversion of hyperpolarized L-[1-13C]Lactate into Pyruvate has been observed in vitro using lactate dehydrogenase (LDH) and in a cells lysate. From the acquisition of a series of 13C-NMR spectra, the metabolic build-up of the [1-13C]Pyruvate signal has been observed. These studies demonstrate that, even if the experimental set-up used for these PHIP-SAH hyperpolarization studies is still far from optimal, the attained polarization level is already sufficient to carry out in vitro metabolic studies.
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5
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1204
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-3765
lactate dehydrogenase; metabolism; NMR spectroscopy; pyruvates;
Cavallari, Eleonora; Carrera, Carla; Aime, Silvio; Reineri, Francesca
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1619402
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