The purpose of this studywas to develop a simple, direct,multiresidue highly specific procedure to evaluate the possible surface contamination of selected antineoplastic drugs in several hospital environment sites by using wipe test sampling. 5-fluorouracil (5-FU), carboplatin (C-Pt), cyclophosphamide (CYC), cytarabine (CYT), doxorubicin (DOX), gemcitabine (GEM), ifosfamide (IFO),methotrexate (MET), and mitomycin C (MIT) belong to very different chemical classes but show good ionization properties under electrospray ionization (ESI) conditions (negative ion mode for 5-FU and positive ion mode in all other cases). HPLC (high performance liquid chromatography) coupled with HRMS (high resolution mass spectrometry) appears to be the best technique for direct analysis of these analytes, because neither derivatization nor complex extraction procedure for polar compounds in samples is requested prior the analysis. Sample preparationwas limited towashing wipeswith appropriate solvents. Chromatographic separationwas achieved on C18 reversed phase columns. The HPLC-HRMS/MS method was validated in order to obtain robustness, sensitivity and selectivity. LLOQ (lower limit of quantitation) values provided a sensitivity good enough to evidence the presence of the drugs in a very low concentration range (<1pg/cm2). The method was applied for a study of real wipe tests coming from many areas from a hospital showing some positive samples. The low quantitation limits and the high specificity due to the high resolution approach of the developed method allowed an accurate description of the working environment that can be used to define procedural rules to limit working place contamination to a minimum.

Antineoplastic drugs determination by HPLC-HRMSn to monitor occupational exposure

DAL BELLO, FEDERICA;SANTORO, VALENTINA;SCARPINO, VALENTINA;MARTANO, CHIARA;AIGOTTI, Riccardo;MEDANA, Claudio
Last
2016-01-01

Abstract

The purpose of this studywas to develop a simple, direct,multiresidue highly specific procedure to evaluate the possible surface contamination of selected antineoplastic drugs in several hospital environment sites by using wipe test sampling. 5-fluorouracil (5-FU), carboplatin (C-Pt), cyclophosphamide (CYC), cytarabine (CYT), doxorubicin (DOX), gemcitabine (GEM), ifosfamide (IFO),methotrexate (MET), and mitomycin C (MIT) belong to very different chemical classes but show good ionization properties under electrospray ionization (ESI) conditions (negative ion mode for 5-FU and positive ion mode in all other cases). HPLC (high performance liquid chromatography) coupled with HRMS (high resolution mass spectrometry) appears to be the best technique for direct analysis of these analytes, because neither derivatization nor complex extraction procedure for polar compounds in samples is requested prior the analysis. Sample preparationwas limited towashing wipeswith appropriate solvents. Chromatographic separationwas achieved on C18 reversed phase columns. The HPLC-HRMS/MS method was validated in order to obtain robustness, sensitivity and selectivity. LLOQ (lower limit of quantitation) values provided a sensitivity good enough to evidence the presence of the drugs in a very low concentration range (<1pg/cm2). The method was applied for a study of real wipe tests coming from many areas from a hospital showing some positive samples. The low quantitation limits and the high specificity due to the high resolution approach of the developed method allowed an accurate description of the working environment that can be used to define procedural rules to limit working place contamination to a minimum.
2016
8
7
730
737
http://www3.interscience.wiley.com/journal/121408477/home/ProductInformation.html
antineoplastic; drugs; high resolution tandem mass spectrometry; hospital safety; HPLC; Analytical Chemistry; Environmental Chemistry; 3003; Spectroscopy
Dal Bello, Federica; Santoro, Valentina; Scarpino, Valentina; Martano, Chiara; Aigotti, Riccardo; Chiappa, Alberta; Davoli, Enrico; Medana, Claudio...espandi
File in questo prodotto:
File Dimensione Formato  
dta1827.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 672.29 kB
Formato Adobe PDF
672.29 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1622682
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 15
social impact