A method for preparing solid lipid nanoparticles containing antibodies, comprising the steps of: (a) preparing a micellar solution comprising: from 0.02 mg/ml to 10 mg/ml of at least one anionic surfactant selected from the group consisting of alkylphosphates, alkylsulfates and alkylsulfonates; from 0.1% to 30% by weight on the total weight of the micellar solution of at least one alkaline soap; from 0.1% to 30% by weight on the total weight of the micellar solution of at least one non-ionic amphiphilic polymer selected from the group consisting of partially hydrolyzed polyvinyl acetate, polyoxyethylene/polyoxypropylene copolymers, polyacrylamides, polyvinylpyrrolidone and derivatives thereof, dextrane derivatives and agarose derivatives with various molecular weights, cellulose derivatives, non-ionic rubbers, cyclodextrins and derivatives thereof; said micellar solution being prepared by means of the following steps: (i) solubilizing in water the at least one anionic surfactant; (ii) adding the at least one alkaline soap, heating to a temperature comprised between 35°C and 90°C; and (iii) adding the at least one non-ionic amphiphilic polymer; (b) adding to the micellar solution prepared in step (a) a solution that contains at least one antibody of interest at a concentration comprised between 0.1 mg/ml and 10 mg/ml with respect to the final solution resulting from step (b); (c) adding to the solution obtained in step (b) at least one acid at a concentration comprised between 0.01M and 5M with respect to the final solution resulting from step (c); (d) cooling in an ice bath the suspension obtained in step (c) down to ambient temperature.
Method for preparing solid lipid nanoparticles containing antibodies in ion pair form using the fatty acid coacervation technique
GALLARATE, Marina;PEIRA, Elena;BATTAGLIA, Luigi Sebastiano
2015-01-01
Abstract
A method for preparing solid lipid nanoparticles containing antibodies, comprising the steps of: (a) preparing a micellar solution comprising: from 0.02 mg/ml to 10 mg/ml of at least one anionic surfactant selected from the group consisting of alkylphosphates, alkylsulfates and alkylsulfonates; from 0.1% to 30% by weight on the total weight of the micellar solution of at least one alkaline soap; from 0.1% to 30% by weight on the total weight of the micellar solution of at least one non-ionic amphiphilic polymer selected from the group consisting of partially hydrolyzed polyvinyl acetate, polyoxyethylene/polyoxypropylene copolymers, polyacrylamides, polyvinylpyrrolidone and derivatives thereof, dextrane derivatives and agarose derivatives with various molecular weights, cellulose derivatives, non-ionic rubbers, cyclodextrins and derivatives thereof; said micellar solution being prepared by means of the following steps: (i) solubilizing in water the at least one anionic surfactant; (ii) adding the at least one alkaline soap, heating to a temperature comprised between 35°C and 90°C; and (iii) adding the at least one non-ionic amphiphilic polymer; (b) adding to the micellar solution prepared in step (a) a solution that contains at least one antibody of interest at a concentration comprised between 0.1 mg/ml and 10 mg/ml with respect to the final solution resulting from step (b); (c) adding to the solution obtained in step (b) at least one acid at a concentration comprised between 0.01M and 5M with respect to the final solution resulting from step (c); (d) cooling in an ice bath the suspension obtained in step (c) down to ambient temperature.File | Dimensione | Formato | |
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