Flow cytometry is a useful tool for diagnosis and minimal residual disease (MRD) study of hematological diseases. Standard sample preparation protocols are characterized by stain-lyse-wash (SLW). To prevent nonspecific bindings and achieve high sensitivity in MRD studies, lyse-wash-stain-wash (LWSW) is required. To our knowledge, no comparison between the two methods has been performed.
Relevance of sample preparation for flow cytometry
SARACI, ENI;ODDOLO, DANIELA;RUGGERI, Marina;CALTAGIRONE, Simona Angela;BRUNO, Benedetto;BOCCADORO, Mario;OMEDE', Pierluigi
2018-01-01
Abstract
Flow cytometry is a useful tool for diagnosis and minimal residual disease (MRD) study of hematological diseases. Standard sample preparation protocols are characterized by stain-lyse-wash (SLW). To prevent nonspecific bindings and achieve high sensitivity in MRD studies, lyse-wash-stain-wash (LWSW) is required. To our knowledge, no comparison between the two methods has been performed.
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