Among all materials suitable for the fabrication of inert electrodes (Type-0) for amperometry, diamond is the one that shows the best pros/cons ratio. It is in fact very sensitive, chemically inert, biocompatible and transparent. This latter property was used so far only for cell inspection and not for optical measurements. In this work, we demonstrate for the first time the suitability of diamond MEAs for simultaneously detecting amperometric and fluorescence signals associated with calcium transients and catecholamine release in in vitro chromaffin cells. This is now possible thanks to the recently improved technology of the diamond MEAs, which allows to reach transmittance of ~ 50% in the whole optical range of interest, while keeping excellent amperometric sensitivity.

Simultaneous Fluorescent and Amperometric Detection Of Catecholamine Release From Neuroendocrine Cells With Transparent Diamond MEAs

Alberto, Pasquarelli;Andrea, Marcantoni;Daniela, Gavello;Alfio, Battiato;Federico, Picollo;Paolo, Olivero;Emilio, Carbone;Valentina, Carabelli
2016-01-01

Abstract

Among all materials suitable for the fabrication of inert electrodes (Type-0) for amperometry, diamond is the one that shows the best pros/cons ratio. It is in fact very sensitive, chemically inert, biocompatible and transparent. This latter property was used so far only for cell inspection and not for optical measurements. In this work, we demonstrate for the first time the suitability of diamond MEAs for simultaneously detecting amperometric and fluorescence signals associated with calcium transients and catecholamine release in in vitro chromaffin cells. This is now possible thanks to the recently improved technology of the diamond MEAs, which allows to reach transmittance of ~ 50% in the whole optical range of interest, while keeping excellent amperometric sensitivity.
2016
Front. Neurosci. Conference Abstract: MEA Meeting 2016 - 10th International Meeting on Substrate-Integrated Electrode Arrays
Reutlingen, Germania
28/06/2016 - 01/07/2016
Proceedings of Front. Neurosci. Conference Abstract: MEA Meeting 2016 - 10th International Meeting on Substrate-Integrated Electrode Arrays
Front. Neurosci. Conference Abstract: MEA Meeting 2016 - 10th International Meeting on Substrate-Integrated Electrode Arrays
10
1
2
http://www.frontiersin.org/10.3389/conf.fnins.2016.93.00129/event_abstract
Alberto, Pasquarelli; Andrea, Marcantoni; Daniela, Gavello; Alfio, Battiato; Federico, Picollo; Paolo, Olivero; Emilio, Carbone; Valentina, Carabelli...espandi
File in questo prodotto:
File Dimensione Formato  
articolo_online.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 159.91 kB
Formato Adobe PDF
159.91 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1660588
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact