Among all materials suitable for the fabrication of inert electrodes (Type-0) for amperometry, diamond is the one that shows the best pros/cons ratio. It is in fact very sensitive, chemically inert, biocompatible and transparent. This latter property was used so far only for cell inspection and not for optical measurements. In this work, we demonstrate for the first time the suitability of diamond MEAs for simultaneously detecting amperometric and fluorescence signals associated with calcium transients and catecholamine release in in vitro chromaffin cells. This is now possible thanks to the recently improved technology of the diamond MEAs, which allows to reach transmittance of ~ 50% in the whole optical range of interest, while keeping excellent amperometric sensitivity.

Simultaneous Fluorescent and Amperometric Detection Of Catecholamine Release From Neuroendocrine Cells With Transparent Diamond MEAs

Alberto, Pasquarelli;Andrea, Marcantoni;Daniela, Gavello;Alfio, Battiato;Federico, Picollo;Paolo, Olivero;Emilio, Carbone;Valentina, Carabelli
2016

Abstract

Among all materials suitable for the fabrication of inert electrodes (Type-0) for amperometry, diamond is the one that shows the best pros/cons ratio. It is in fact very sensitive, chemically inert, biocompatible and transparent. This latter property was used so far only for cell inspection and not for optical measurements. In this work, we demonstrate for the first time the suitability of diamond MEAs for simultaneously detecting amperometric and fluorescence signals associated with calcium transients and catecholamine release in in vitro chromaffin cells. This is now possible thanks to the recently improved technology of the diamond MEAs, which allows to reach transmittance of ~ 50% in the whole optical range of interest, while keeping excellent amperometric sensitivity.
Front. Neurosci. Conference Abstract: MEA Meeting 2016 - 10th International Meeting on Substrate-Integrated Electrode Arrays
Reutlingen, Germania
28/06/2016 - 01/07/2016
Proceedings of Front. Neurosci. Conference Abstract: MEA Meeting 2016 - 10th International Meeting on Substrate-Integrated Electrode Arrays
Front. Neurosci. Conference Abstract: MEA Meeting 2016 - 10th International Meeting on Substrate-Integrated Electrode Arrays
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http://www.frontiersin.org/10.3389/conf.fnins.2016.93.00129/event_abstract
Alberto, Pasquarelli; Andrea, Marcantoni; Daniela, Gavello; Alfio, Battiato; Federico, Picollo; Paolo, Olivero; Emilio, Carbone; Valentina, Carabelli
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1660588
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