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EnglishFollowing herbivore attacks, plants modify a blend of volatiles organic compounds (VOCs) released, resulting in the attraction of their antagonists. However, volatiles released constitutively may affect herbivores and natural enemies’ fitness too. In tomato there is still a lack of information on the genetic bases responsible for the constitutive release of VOC involved in direct and indirect defenses. Here we studied the constitutive emissions related to the two most abundant sesquiterpene synthase genes expressed in tomato and their functional role in plant defense. Using an RNA interference approach, we silenced the expression of TPS9 and TPS12 genes and assessed the effect of this transformation on herbivores and parasitoids. We found that silenced plants displayed a different constitutive volatiles emission from controls, resulting in reduced attractiveness for the aphid parasitoid Aphidius ervi and in an impaired development of Spodoptera exigua larvae. We discussed these data considering the transcriptional regulation of key-genes involved in the pathway of VOC metabolism. We provide several lines of evidence on the metabolic flux from terpenoids to phenylpropanoids. Our results shed more light on constitutive defenses mediated by plant volatiles and on the molecular mechanisms involved in their metabolic regulation
| Titolo: | TPS Genes Silencing Alters Constitutive Indirect and Direct Defense in Tomato |
| Autori Riconosciuti: | |
| Autori: | Mariangela Coppola, Pasquale Cascone, Simone Bossi, Giandomenico Corrado, Antonio Pietro Garonna, Massimo Maffei, Rosa Rao, Emilio Guerrieri |
| Data di pubblicazione: | 2018 |
| Abstract: | Following herbivore attacks, plants modify a blend of volatiles organic compounds (VOCs) released, resulting in the attraction of their antagonists. However, volatiles released constitutively may affect herbivores and natural enemies’ fitness too. In tomato there is still a lack of information on the genetic bases responsible for the constitutive release of VOC involved in direct and indirect defenses. Here we studied the constitutive emissions related to the two most abundant sesquiterpene synthase genes expressed in tomato and their functional role in plant defense. Using an RNA interference approach, we silenced the expression of TPS9 and TPS12 genes and assessed the effect of this transformation on herbivores and parasitoids. We found that silenced plants displayed a different constitutive volatiles emission from controls, resulting in reduced attractiveness for the aphid parasitoid Aphidius ervi and in an impaired development of Spodoptera exigua larvae. We discussed these data considering the transcriptional regulation of key-genes involved in the pathway of VOC metabolism. We provide several lines of evidence on the metabolic flux from terpenoids to phenylpropanoids. Our results shed more light on constitutive defenses mediated by plant volatiles and on the molecular mechanisms involved in their metabolic regulation |
| Volume: | 19 |
| Fascicolo: | 9 |
| Pagina iniziale: | 1 |
| Pagina finale: | 19 |
| Digital Object Identifier (DOI): | 10.3390/ijms19092748 |
| URL: | https://www.mdpi.com/1422-0067/19/9/2748 |
| Parole Chiave: | constitutive release of VOC; Sesquiterpene Synthase; RNAi; Aphidius ervi; Spodoptera exigua |
| Rivista: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| 2018 TPS and Tomato IJMS.pdf | Articolo principale | 2 PDF Editoriale | Open Access Visualizza/Apri |
http://hdl.handle.net/2318/1677338
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