Motivation: A basic question in protein science is to which extent mutations affect protein thermostability. This knowledge would be particularly relevant for engineering thermostable enzymes. In several experimental approaches, this issue has been serendipitously addressed.It would be therefore convenient providing a computational method that predicts when a given protein mutant is more thermostable than its corresponding wild-type. Results: We present a new method based on support vector machines that is able to predict if a set of mutations (including insertion and deletions) can enhance the thermostability of a given protein sequence. When trained and tested on a redundancy-reduced dataset, our predictor achieves 88% accuracy and a correlation coefficient equal to 0.75. Our predictor also correctly classifes 12 out of 14 experimentally characterized protein mutants with enhanced thermostability. Finally, it correctly detects all the 11 mutated proteins whose increase in stability temperature is Availability: The dataset and the list of protein clusters adopted for the SVM cross-validation are available at the web site

Predicting protein thermostability changes from sequence upon multiple mutations

Fariselli P.;
2008

Abstract

Motivation: A basic question in protein science is to which extent mutations affect protein thermostability. This knowledge would be particularly relevant for engineering thermostable enzymes. In several experimental approaches, this issue has been serendipitously addressed.It would be therefore convenient providing a computational method that predicts when a given protein mutant is more thermostable than its corresponding wild-type. Results: We present a new method based on support vector machines that is able to predict if a set of mutations (including insertion and deletions) can enhance the thermostability of a given protein sequence. When trained and tested on a redundancy-reduced dataset, our predictor achieves 88% accuracy and a correlation coefficient equal to 0.75. Our predictor also correctly classifes 12 out of 14 experimentally characterized protein mutants with enhanced thermostability. Finally, it correctly detects all the 11 mutated proteins whose increase in stability temperature is Availability: The dataset and the list of protein clusters adopted for the SVM cross-validation are available at the web site
16th Annual International Conference Intelligent Systems for Molecular Biology- ISMB2008
TORONTO
19-23/7/2008
16th Annual International Conference Intelligent Systems for Molecular Biology- ISMB2008
94
94
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1687493
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