Proteins are essential constituents of living organisms. The increased development of fluorescent probes with proper selectivity offers opportunities to explore the roles of proteins in physiological functions. Squaraine dyes have demonstrated to be valuable fluorescent probes given their ability to turn-on their fluorescence in response to specific proteins. Here we investigate the binding of four different squaraines with commercially available transferrin, fibrinogen, trypsin, pepsin and a generic protease. The protein-dye interaction was studied by means of UV–Vis and fluorescence spectroscopy. The association (KA) and dissociation (KD) constants were determined based on the turn-on response of squaraines in presence of the proteins, and the quantum yield of the complexes was measured. The protein's surface hydrophobicity seems to play an important role on the fluorescence turn-on response of the squaraines, especially for those with shorter alkyl chains.

Interaction of squaraine dyes with proteins: Looking for more efficient fluorescent turn-on probes

Butnarasu C.
First
;
Barbero N.
;
Barolo C.;Visentin S.
Last
2021-01-01

Abstract

Proteins are essential constituents of living organisms. The increased development of fluorescent probes with proper selectivity offers opportunities to explore the roles of proteins in physiological functions. Squaraine dyes have demonstrated to be valuable fluorescent probes given their ability to turn-on their fluorescence in response to specific proteins. Here we investigate the binding of four different squaraines with commercially available transferrin, fibrinogen, trypsin, pepsin and a generic protease. The protein-dye interaction was studied by means of UV–Vis and fluorescence spectroscopy. The association (KA) and dissociation (KD) constants were determined based on the turn-on response of squaraines in presence of the proteins, and the quantum yield of the complexes was measured. The protein's surface hydrophobicity seems to play an important role on the fluorescence turn-on response of the squaraines, especially for those with shorter alkyl chains.
2021
184
108873
108873
Binding; Fluorescence; Protein; Squaraine; Turn-on; UV–Vis
Butnarasu C.; Barbero N.; Barolo C.; Visentin S.
File in questo prodotto:
File Dimensione Formato  
2021 D&P.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 3.68 MB
Formato Adobe PDF
3.68 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
SQ-Proteins_8giu20.docx

Accesso aperto

Tipo di file: PREPRINT (PRIMA BOZZA)
Dimensione 1.51 MB
Formato Microsoft Word XML
1.51 MB Microsoft Word XML Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1760401
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 19
social impact