Hyperpolarization-enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13 C, 15 N, or 129 Xe due to their long spin-polarization lifetimes and the absence of a proton-background signal from water and fat in the images. Here we present a novel type of 1 H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long-lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para -enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D 2 O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water. The hyperpolarization-enhanced 1 H-imaging modality presented here can allow for hyperpolarized imaging without the need for low-abundance, low-sensitivity heteronuclei.

Singlet-contrast magnetic resonance imaging: unlocking hyperpolarization with metabolism

Cavallari, Eleonora;Carrera, Carla;Aime, Silvio;Reineri, Francesca;
2020

Abstract

Hyperpolarization-enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13 C, 15 N, or 129 Xe due to their long spin-polarization lifetimes and the absence of a proton-background signal from water and fat in the images. Here we present a novel type of 1 H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long-lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para -enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D 2 O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water. The hyperpolarization-enhanced 1 H-imaging modality presented here can allow for hyperpolarized imaging without the need for low-abundance, low-sensitivity heteronuclei.
ANGEWANDTE CHEMIE. INTERNATIONAL EDITION
1
6
Hyperpolarization; MRI; Metabolism; NMR
Eills, James; Cavallari, Eleonora; Kircher, Raphael; Di Matteo, Ginevra; Carrera, Carla; Dagys, Laurynas; Levitt, Malcolm H; Ivanov, Konstantin L; Aime, Silvio; Reineri, Francesca; Münnemann, Kerstin; Budker, Dmitry; Buntkowsky, Gerd; Knecht, Stephan
File in questo prodotto:
File Dimensione Formato  
1H_imaging_con_copertina.pdf

embargo fino al 01/01/2022

Descrizione: articolo principale
Tipo di file: PREPRINT (PRIMA BOZZA)
Dimensione 983.58 kB
Formato Adobe PDF
983.58 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1766007
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 13
social impact