In our recent studies, we have developed a thermodynamic biochemical model able to select the resonant frequency of an extremely low frequency electromagnetic field (ELF-EMF) specifically affecting different types of cancer, and we have demonstrated its effects in vitro. In this work, we investigate the cellular response to the ELF electromagnetic wave in three-dimensional (3D) culture models, which mimic the features of tumors in vivo. Cell membrane was modelled as a resistor-capacitor circuit and the specific thermal resonant frequency was calculated and tested on two-dimensional (2D) and three-dimensional (3D) cell cultures of human pancreatic cancer, glioblastoma and breast cancer. Cell proliferation and the transcription of respiratory chain and adenosine triphosphate synthase subunits, as well as uncoupling proteins, were assessed. For the first time, we demonstrate that an ELF-EMF hampers growth and potentiates both the coupled and uncoupled respiration of all analyzed models. Interestingly, the metabolic shift was evident even in the 3D aggregates, making this approach particularly valuable and promising for future application in vivo, in aggressive cancer tissues characterized by resistance to treatments.

Thermomagnetic Resonance Effect of the Extremely Low Frequency Electromagnetic Field on Three-Dimensional Cancer Models

Bergandi, Loredana
First
;
Salaroglio, Iris Chiara;Gesmundo, Iacopo;Granata, Riccarda;Ponzetto, Antonio;Silvagno, Francesca
Last
2022-01-01

Abstract

In our recent studies, we have developed a thermodynamic biochemical model able to select the resonant frequency of an extremely low frequency electromagnetic field (ELF-EMF) specifically affecting different types of cancer, and we have demonstrated its effects in vitro. In this work, we investigate the cellular response to the ELF electromagnetic wave in three-dimensional (3D) culture models, which mimic the features of tumors in vivo. Cell membrane was modelled as a resistor-capacitor circuit and the specific thermal resonant frequency was calculated and tested on two-dimensional (2D) and three-dimensional (3D) cell cultures of human pancreatic cancer, glioblastoma and breast cancer. Cell proliferation and the transcription of respiratory chain and adenosine triphosphate synthase subunits, as well as uncoupling proteins, were assessed. For the first time, we demonstrate that an ELF-EMF hampers growth and potentiates both the coupled and uncoupled respiration of all analyzed models. Interestingly, the metabolic shift was evident even in the 3D aggregates, making this approach particularly valuable and promising for future application in vivo, in aggressive cancer tissues characterized by resistance to treatments.
2022
23
14
7955
7973
cancer spheroids; electron transport chain; extremely low frequency electromagnetic field; human breast cancer; human glioblastoma; human pancreatic cancer; mitochondrial respiration; proliferation; thermodynamic approach; uncoupling proteins; Cell Proliferation; Electromagnetic Radiation; Humans; Electromagnetic Fields; Glioblastoma
Bergandi, Loredana; Lucia, Umberto; Grisolia, Giulia; Salaroglio, Iris Chiara; Gesmundo, Iacopo; Granata, Riccarda; Borchiellini, Romano; Ponzetto, Antonio; Silvagno, Francesca
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1872820
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