Background: COVID-19 is characterized by an uncontrolled inflammatory response with high pro-inflammatory cytokine production through the activation of intracellular pathways, such as mitogen-activated protein kinase (MAPK). Viruses are able to exploit the MAPK pathway to their advantage; this pathway relevance to severe COVID-19 is poorly described. The aim of this study was to quantify biomarkers involved in the MAPK pathway and to clarify its possible role in affecting some COVID-19-related clinical features. Methods: H-RAS, C-RAF, MAPK1, MAPK2, and ERK were quantified through ELISA, and genetic polymorphisms were evaluated through real-time PCR. Results: We prospectively recruited 201 individuals (158 positive and 43 negative for SARS-CoV-2): 35 were male, and their median age was 65 years. MAPK-related biomarker levels were increased in SARS-CoV-2-positive participants (n = 89) compared to negative ones (n = 29). Dyspnea was reported by 48%; this symptom was associated with PBMC C-RAF levels in positive participants (p = 0.022) and type of ventilation (p = 0.031). The highest degree of ventilation was used by 8% for invasive ventilation and 41% for continuous positive airway pressure (CPAP). Conclusions: This is the first study that showed a possible contribution of MAPK-related biomarkers in affecting COVID-19 clinical features, and this may be relevant for identifying COVID-19 positive participants at risk of serious complications.

COVID-19: A Possible Contribution of the MAPK Pathway

Cusato, Jessica
Co-first
;
Manca, Alessandra
Co-first
;
Palermiti, Alice
;
Mula, Jacopo
;
Costanzo, Martina;Antonucci, Miriam;Trunfio, Mattia;Corcione, Silvia;De Vivo, Elisa Delia;Ianniello, Alice;Ferrara, Micol;Di Perri, Giovanni;De Rosa, Francesco Giuseppe;D'Avolio, Antonio
Co-last
;
Calcagno, Andrea
Co-last
2023-01-01

Abstract

Background: COVID-19 is characterized by an uncontrolled inflammatory response with high pro-inflammatory cytokine production through the activation of intracellular pathways, such as mitogen-activated protein kinase (MAPK). Viruses are able to exploit the MAPK pathway to their advantage; this pathway relevance to severe COVID-19 is poorly described. The aim of this study was to quantify biomarkers involved in the MAPK pathway and to clarify its possible role in affecting some COVID-19-related clinical features. Methods: H-RAS, C-RAF, MAPK1, MAPK2, and ERK were quantified through ELISA, and genetic polymorphisms were evaluated through real-time PCR. Results: We prospectively recruited 201 individuals (158 positive and 43 negative for SARS-CoV-2): 35 were male, and their median age was 65 years. MAPK-related biomarker levels were increased in SARS-CoV-2-positive participants (n = 89) compared to negative ones (n = 29). Dyspnea was reported by 48%; this symptom was associated with PBMC C-RAF levels in positive participants (p = 0.022) and type of ventilation (p = 0.031). The highest degree of ventilation was used by 8% for invasive ventilation and 41% for continuous positive airway pressure (CPAP). Conclusions: This is the first study that showed a possible contribution of MAPK-related biomarkers in affecting COVID-19 clinical features, and this may be relevant for identifying COVID-19 positive participants at risk of serious complications.
2023
11
5
1459
1469
PBMCs; SARS-CoV-2; inflammation mechanism; molecular pathway; oxygenation
Cusato, Jessica; Manca, Alessandra; Palermiti, Alice; Mula, Jacopo; Costanzo, Martina; Antonucci, Miriam; Trunfio, Mattia; Corcione, Silvia; Chiara, Francesco; De Vivo, Elisa Delia; Ianniello, Alice; Ferrara, Micol; Di Perri, Giovanni; De Rosa, Francesco Giuseppe; D'Avolio, Antonio; Calcagno, Andrea
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1906432
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