L'impiego di differenti medium nella produzione in vitro di embrioni di gatto domestico (Felis Catus) in vitro production of cat embryos using different media

The domestic cat is an animal model for the study of the wild felines reproduction. The degree of efficiency of ART in this specie is generally inferior to other species. It stands to reason that the improvement of the culture condition is very important for the development of the ART in the felids. This study values the effect of two different medium on the efficiency of maturation in vitro of domestic cat oocytes and on following ability of embryonal development after fecondation in vitro. The IVM has been effected in two different medium MEM and TCM199 (Sigma Aldrich srl). After the culture in vitro the COCs have been subjected to colouring with Lacmoid (Sigma Aldrich srl) to value the nuclear maturation and to Fecundation in Vitro (FIV) and to Culture in Vitro (IVC) to value the ability of embryonal development. It is evident on the ground of the results that the employment of the TCM 199 in the maturation in vitro of cat oocytes gives better results than the MEM.