Embryo development is dependent upon the exchange of oxygen and nutrients through the placenta, mainly composed of peculiar epithelioid cells, known as trophoblast cells. Normal trophoblast functionality plays a key role during the whole pregnancy, especially in the first stage of placentation. This chapter explains the techniques to obtain sheep primary trophoblast cells from the early placenta. Overall, procedures for cell isolation, culture, characterization, and cryopreservation are described.

Ovine Trophoblast Cells: Cell Isolation and Culturing from the Placenta at the Early Stage of Pregnancy

Paola Toschi
;
Irene Viola;Isabella Manenti;Silvia Miretti;Elisabetta Macchi;Eugenio Martignani;Paolo Accornero;Mario Baratta
2024-01-01

Abstract

Embryo development is dependent upon the exchange of oxygen and nutrients through the placenta, mainly composed of peculiar epithelioid cells, known as trophoblast cells. Normal trophoblast functionality plays a key role during the whole pregnancy, especially in the first stage of placentation. This chapter explains the techniques to obtain sheep primary trophoblast cells from the early placenta. Overall, procedures for cell isolation, culture, characterization, and cryopreservation are described.
2024
Epithelial Cell Culture. Methods in Molecular Biology
Mario Baratta
2749
123
133
978-1-0716-3609-1
Early placenta Primary trophoblast cells Sheep Cell isolation Morphology
Paola Toschi, Irene Viola, Isabella Manenti, Silvia Miretti, Elisabetta Macchi, Eugenio Martignani, Paolo Accornero, Mario Baratta
File in questo prodotto:
File Dimensione Formato  
2024_Ovine Trophoblast Cells Cell Isolation and Culturing.pdf

Accesso riservato

Descrizione: Ovine Trophoblast Cells Cell Isolation and Culturing.
Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 468.25 kB
Formato Adobe PDF
468.25 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1950691
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact