Spermatozoa were collected from cat epididymes after routine castration and diluted into one of two extenders containing a final concentration of 20% egg yolk, 5% glycerol and either fructose or glucose. Straws were frozen over liquid nitrogen. Neither the decreases in motility (optic microscope) and in viability (eosin-nigrosin stain) before freezing and at thawing, nor the longevity of spermatozoa incubated at 38°C, showed differences between the two extenders. Motility appeared to decrease at a slower rate with fructose, but this observation needs further confirmation.

Confronto tra due diluitori per il congelamento di spermatozoi epididimari di gatto

ROTA, Ada;STARVAGGI CUCUZZA, Alessandro;
2006

Abstract

Spermatozoa were collected from cat epididymes after routine castration and diluted into one of two extenders containing a final concentration of 20% egg yolk, 5% glycerol and either fructose or glucose. Straws were frozen over liquid nitrogen. Neither the decreases in motility (optic microscope) and in viability (eosin-nigrosin stain) before freezing and at thawing, nor the longevity of spermatozoa incubated at 38°C, showed differences between the two extenders. Motility appeared to decrease at a slower rate with fructose, but this observation needs further confirmation.
IV Congresso Nazionale S.I.R.A.
Pisa
8-10 giugno 2006
Atti S.I.R.A.
S.I.R.A.
IV
78
80
cat; freezing; epidydimal spermatozoa.
ROTA A; STARVAGGI CUCUZZA A; LACERENZA D; MILANI C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/19710
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