The coordinate modulation of cadherin and integrin functions plays an essential role in fundamental physiological and pathological processes, including morphogenesis and cancer. However, the molecular mechanisms underlying the functional crosstalk between cadherins and integrins are still elusive. We show that the small GTPase Rap1, a crucial regulator of the inside-out activation of integrins, is a target for E-cadherin-mediated outside-in signaling. In particular, a strong activation of Rap1 occurs upon adherens junction disassembly and is triggered by E-cadherin internalization and trafficking along the endocytic pathway. In contrast, Rap1 activity is not influenced by integrin outside-in signaling. Moreover, the activation of Rap1 is associated with and controlled by an increased Src kinase activity, and is paralleled by the colocalization of Rap1 and E-cadherin at the perinuclear recycling endosome compartment, and the association of Rap1 with a subset of E-cadherin-catenin complexes that does not contain p120ctn. Finally, the E-cadherin endocytosis-dependent activation of Rap1 is associated with and is required for the formation of integrin-based focal adhesions. Our findings provide the first evidence of an E-cadherin-modulated endosomal signaling pathway involving Rap1, and suggest that cadherins may have a novel modulatory role in integrin adhesive functions by fine-tuning Rap1 activation.

Rap1: a turnabout for the crossroad between cadherin and integrin signaling

BALZAC, Fiorella;AVOLIO, Maria;DEGANI, Simona;FRANCALANCI, FLORIANA;TARONE, Guido;RETTA, Saverio Francesco
2006-01-01

Abstract

The coordinate modulation of cadherin and integrin functions plays an essential role in fundamental physiological and pathological processes, including morphogenesis and cancer. However, the molecular mechanisms underlying the functional crosstalk between cadherins and integrins are still elusive. We show that the small GTPase Rap1, a crucial regulator of the inside-out activation of integrins, is a target for E-cadherin-mediated outside-in signaling. In particular, a strong activation of Rap1 occurs upon adherens junction disassembly and is triggered by E-cadherin internalization and trafficking along the endocytic pathway. In contrast, Rap1 activity is not influenced by integrin outside-in signaling. Moreover, the activation of Rap1 is associated with and controlled by an increased Src kinase activity, and is paralleled by the colocalization of Rap1 and E-cadherin at the perinuclear recycling endosome compartment, and the association of Rap1 with a subset of E-cadherin-catenin complexes that does not contain p120ctn. Finally, the E-cadherin endocytosis-dependent activation of Rap1 is associated with and is required for the formation of integrin-based focal adhesions. Our findings provide the first evidence of an E-cadherin-modulated endosomal signaling pathway involving Rap1, and suggest that cadherins may have a novel modulatory role in integrin adhesive functions by fine-tuning Rap1 activation.
2006
Cell Signaling World 2006: Signal Transduction Pathways as Therapeutic Targets
European Conference Center, Kirchberg – Luxembourg
25-28 Gennaio 2006
Proceeding of "Cell Signaling World 2006: Signal Transduction Pathways as Therapeutic Targets"
Marc Diederich
-
130
130
http://www.transduction-meeting.lu
Cadherins; Integrins; Rap1; Molecular crosstalk
BALZAC F; AVOLIO M; DEGANI S; FRANCALANCI F; KAVERINA I; TARONE G; SMALL V; RETTA S
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/19948
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact