A representative number of tomato landraces collected in the main Italian tomato growing areas belonging to different typologies were evaluated against Fusarium oxysporum f. sp. lycopersici and Verticillium dahliae by root dip inoculation method. None of the screened landraces was completely resistant to Fusarium wilt, nevertheless high levels of tolerance were found in ‘Ovalino’, ‘Locale Valle d’Agri’, ‘Patataro pop.29’(a typical accession from Campania), ‘Sorrentino’ (a pink tomato selected in the Sorrento area) and ‘Mezzo lungo’ and intermediate in some other 11 landraces. Similarly most of the genotypes were highly susceptible to V. dahliae, but ‘Eraldo 4’, belonging to Cuore di Bue di Albenga type, ‘Rosa di Rotonda’ and ‘Locale Valle d’Agri’ (an accessions with pink fruit from Basilicata) and the breeding line ‘L 16-94’, appeared to be tolerant, showing disease incidence ranging 72.2-100% and disease severity 26.6 – 30%, whereas ‘Sorrentino’ was as resistant as ‘Florida’. Two new molecular markers linked to Verticillium resistance genes were developed on the sequence information in GenBank for Ve genes and validated on several resistant varieties. Genetic analysis carried out using these markers demonstrated that the same resistant alleles are present both in ‘Sorrentino’ and ‘Eraldo4’. Two years of individual selection and self-fertilization gave rise some breeding lines of ‘Sorrentino’ resistant to V. dahliae and highly tolerant to F. oxysporum. Eraldo 4 is currently used as parent for the production of resistant and typical HF1 hybrids of the “Cuor di Bue di Albenga” which is one of the Italian most popular typology for fresh consumption.
Identification of resistant traits to Fusarium and Verticillium wilt in Italian tomato landraces
VALENTINO, Danila;TAMIETTI, Giacomo
2007-01-01
Abstract
A representative number of tomato landraces collected in the main Italian tomato growing areas belonging to different typologies were evaluated against Fusarium oxysporum f. sp. lycopersici and Verticillium dahliae by root dip inoculation method. None of the screened landraces was completely resistant to Fusarium wilt, nevertheless high levels of tolerance were found in ‘Ovalino’, ‘Locale Valle d’Agri’, ‘Patataro pop.29’(a typical accession from Campania), ‘Sorrentino’ (a pink tomato selected in the Sorrento area) and ‘Mezzo lungo’ and intermediate in some other 11 landraces. Similarly most of the genotypes were highly susceptible to V. dahliae, but ‘Eraldo 4’, belonging to Cuore di Bue di Albenga type, ‘Rosa di Rotonda’ and ‘Locale Valle d’Agri’ (an accessions with pink fruit from Basilicata) and the breeding line ‘L 16-94’, appeared to be tolerant, showing disease incidence ranging 72.2-100% and disease severity 26.6 – 30%, whereas ‘Sorrentino’ was as resistant as ‘Florida’. Two new molecular markers linked to Verticillium resistance genes were developed on the sequence information in GenBank for Ve genes and validated on several resistant varieties. Genetic analysis carried out using these markers demonstrated that the same resistant alleles are present both in ‘Sorrentino’ and ‘Eraldo4’. Two years of individual selection and self-fertilization gave rise some breeding lines of ‘Sorrentino’ resistant to V. dahliae and highly tolerant to F. oxysporum. Eraldo 4 is currently used as parent for the production of resistant and typical HF1 hybrids of the “Cuor di Bue di Albenga” which is one of the Italian most popular typology for fresh consumption.
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