Three clones containing vertebrate histone genes, two from chicken and one from human have been investigated. DNA from each recombinant within lambda Charon 4A has been strand-separated on agarose gels in the presence of poly r(UG). Southern transfer analysis with histone cDNA indicates that in each case transcription occurs from both strands. These results argue against the possibility of polycistronic transcription of these vertebrate histone genes.
Vertebrate histone gene transcription occurs from both DNA strands.
BRUSCHI, Stefano;
1981-01-01
Abstract
Three clones containing vertebrate histone genes, two from chicken and one from human have been investigated. DNA from each recombinant within lambda Charon 4A has been strand-separated on agarose gels in the presence of poly r(UG). Southern transfer analysis with histone cDNA indicates that in each case transcription occurs from both strands. These results argue against the possibility of polycistronic transcription of these vertebrate histone genes.
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