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English1. Preparative Isoelectric focusing (PIEF) was used to isolate hydroxylasic and dehydrogenasic activities, at different pI. 2. The fraction at pI 4.7 and 4.9 displays a pure dehydrogenase activity (substrate L-DOPA). 3. This fraction did not react with tyrosine, either in the spot-test or in absorption spectra (200-620 nm), and did not exhibit any oxygen consumption. 4. The fraction at pI 4.1 and 4.3 reacted with both L-DOPA and tyrosine as substrate, showing dehydrogenase and hydroxylase activity. 5. The latter activity was confirmed by the oxygen consumption test, showing that molecular oxygen is used to ortho-hydroxylate tyrosine.
| Titolo: | THE AGARICUS BISPORUS TYROSINASE 2: CHARACTERIZATION OF HYDROXYLASE AND DEHYDROGENASE ACTIVITIES. | |
| Autori Riconosciuti: | ||
| Autori: | PAPA G; E. PESSIONE; POLIMENI C; LEONE V; GIUNTA C | |
| Data di pubblicazione: | 1994 | |
| Abstract: | 1. Preparative Isoelectric focusing (PIEF) was used to isolate hydroxylasic and dehydrogenasic activities, at different pI. 2. The fraction at pI 4.7 and 4.9 displays a pure dehydrogenase activity (substrate L-DOPA). 3. This fraction did not react with tyrosine, either in the spot-test or in absorption spectra (200-620 nm), and did not exhibit any oxygen consumption. 4. The fraction at pI 4.1 and 4.3 reacted with both L-DOPA and tyrosine as substrate, showing dehydrogenase and hydroxylase activity. 5. The latter activity was confirmed by the oxygen consumption test, showing that molecular oxygen is used to ortho-hydroxylate tyrosine. | |
| Editore: | Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010 | |
| Volume: | 26 | |
| Pagina iniziale: | 223 | |
| Pagina finale: | 228 | |
| Rivista: | INTERNATIONAL JOURNAL OF BIOCHEMISTRY | |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
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