1. A simplified assay for the flux through the pentose phosphate and the Embden-Meyerhof pathways in the red cell is described. 2. [1-14C]glucose is metabolized to 14CO2 and 14C-anions. 3. 14CO2 is trapped with KOH and residual [14C]glucose and anions are separated by an enzymatic method, which is simpler and quicker than the usual chromatographic method.

A simplified method for the pentose phosphate pathway assay in red cells.

PESCARMONA, Gianpiero;BOSIA, Amalia;GHIGO, Dario Antonio
1982

Abstract

1. A simplified assay for the flux through the pentose phosphate and the Embden-Meyerhof pathways in the red cell is described. 2. [1-14C]glucose is metabolized to 14CO2 and 14C-anions. 3. 14CO2 is trapped with KOH and residual [14C]glucose and anions are separated by an enzymatic method, which is simpler and quicker than the usual chromatographic method.
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PESCARMONA GP ;BOSIA A ;ARESE P ;SARTORI ML ;GHIGO D
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/31150
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