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EnglishThe involvement of CCl4 biotransformation mechanism in decreasing the Protein Kinase C activity has been analyzed in hepatocytes isolated from phenobarbital-pretreated rats. A significant inhibition (55%) and an almost total disappearance (87%) of the enzyme activity were observed at 15 min and at 30 min incubation with CCl4, respectively. Cell preincubation with Trolox or desferrioxamine allowed a marked whilst not complete protection of both cytosolic and particulate Protein Kinase C activity. These results show that the CCl4 reactive metabolites play a primary role in hepatocyte Protein Kinase C impairment and suggest that besides lipid peroxidation other mechanisms -possibly a derangement of Ca2+ homeostasis- may be involved in this process.
| Titolo: | Inactivation of hepatocyte protein kinase C by carbon tetrachloride: involvement of drug's metabolic activation and prooxidant effect. | |
| Autori Riconosciuti: | ||
| Autori: | PRONZATO MA; DOMENICOTTI C; BIASI F; CHIARPOTTO E; COTTALASSO D; VIOTTI P; MELLONI E; MARINARI UM; POLI G | |
| Data di pubblicazione: | 1990 | |
| Abstract: | The involvement of CCl4 biotransformation mechanism in decreasing the Protein Kinase C activity has been analyzed in hepatocytes isolated from phenobarbital-pretreated rats. A significant inhibition (55%) and an almost total disappearance (87%) of the enzyme activity were observed at 15 min and at 30 min incubation with CCl4, respectively. Cell preincubation with Trolox or desferrioxamine allowed a marked whilst not complete protection of both cytosolic and particulate Protein Kinase C activity. These results show that the CCl4 reactive metabolites play a primary role in hepatocyte Protein Kinase C impairment and suggest that besides lipid peroxidation other mechanisms -possibly a derangement of Ca2+ homeostasis- may be involved in this process. | |
| Volume: | 171 | |
| Pagina iniziale: | 1353 | |
| Pagina finale: | 1360 | |
| Rivista: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
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