DNA and isoenzyme analyses were used to characterize twenty table grapevine cultivars including Moscato d’Amburgo, Italia, Sultanina, Bicane and some new varieties recently released. GPI and PGM isoenzyme systems were able to separate the cultivars into nine groups whereas the eight microsatellite loci that were analysed revealed a higher discriminating power. All the cultivars could in fact be distinguished based on the DNA profiles except Sugraone from its sport Sugrafive. Parentage analysis confirmed that the cultivar Italia was obtained crossing Bicane X Moscato d’Amburgo. A difference was observed at one microsatellite locus between Sultanina and the published data for Thompson Seedless, considered its synonym. The different microsatellite loci were evaluated for their informativeness.
Molecular characterization of twenty seeded and seedless table grapevines.
BOTTA, Roberto;
1999-01-01
Abstract
DNA and isoenzyme analyses were used to characterize twenty table grapevine cultivars including Moscato d’Amburgo, Italia, Sultanina, Bicane and some new varieties recently released. GPI and PGM isoenzyme systems were able to separate the cultivars into nine groups whereas the eight microsatellite loci that were analysed revealed a higher discriminating power. All the cultivars could in fact be distinguished based on the DNA profiles except Sugraone from its sport Sugrafive. Parentage analysis confirmed that the cultivar Italia was obtained crossing Bicane X Moscato d’Amburgo. A difference was observed at one microsatellite locus between Sultanina and the published data for Thompson Seedless, considered its synonym. The different microsatellite loci were evaluated for their informativeness.
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