A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true value <6.58%). The method was linear from 0.59 to 150 microg/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.
A Simple and Fast Method for Quantification of Ertapenem using Meropenem as Internal Standard in Human Plasma in a Clinical Setting
D'AVOLIO, ANTONIO
First
;BAIETTO, LORENA;DE ROSA, Francesco Giuseppe;GARAZZINO, Silvia;SCIANDRA, Mauro;SICCARDI, MARCO;BONORA, Stefano;DI PERRI, GiovanniLast
2008-01-01
Abstract
A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true value <6.58%). The method was linear from 0.59 to 150 microg/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.File | Dimensione | Formato | |
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