Cell nuclei were isolated from leaf tissue of different species of Capsicum and the relative fluorescence intensity was measured by flow cytometry after propidium iodide staining. Pea cells with known nuclear genome size (7.09 pg/nucleus) were used as standard to determine the nuclear content of the samples. The found DNA content ranged between 7.65 (C. annuum) and 9.72 pg (C. pubescens), being the general mean of the genus 8.42 pg. According to their DNA content, it was possible to divide the studied species into three main groups. The contribute of the results to a better understanding of systematic relationship within the genus Capsicum is briefly discussed.

Flow cytometric estimation of nuclear DNA content in different species of Capsicum

BELLETTI, Piero;LANTERI, Sergio
1995

Abstract

Cell nuclei were isolated from leaf tissue of different species of Capsicum and the relative fluorescence intensity was measured by flow cytometry after propidium iodide staining. Pea cells with known nuclear genome size (7.09 pg/nucleus) were used as standard to determine the nuclear content of the samples. The found DNA content ranged between 7.65 (C. annuum) and 9.72 pg (C. pubescens), being the general mean of the genus 8.42 pg. According to their DNA content, it was possible to divide the studied species into three main groups. The contribute of the results to a better understanding of systematic relationship within the genus Capsicum is briefly discussed.
Genetics and Breeding on Capsicum and Eggplant
Budapest
19-25 agosto 1995
Proceedings EUCARPIA Meeting on Genetics and Breeding on Capsicum and Eggplant
EUCARPIA
22
25
Flow cytometry; Nucelar DNA content; Capsicum
P. BELLETTI; C. MARZACHI'; E. NADA; S. LANTERI
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/43798
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