Aim of this study was to correlate the human exposure to Formaldehyde (F) with N-methylenvaline, a molecular adduct formed by addiction of F to the N-terminal valine in hemoglobin. A group of 21 subjects employed in a plywood factory and a laminate factory, and occupationally exposed to F, together with a group of 30 controls, were recruited as volunteers to test this biomarker. Each subject received a questionnaire and a passive personal F sampler. Exposure to F vapors and occurrence of N-methylenvaline in blood were measured. Integrated F concentrations always proved lower than Threshold Limit Value as a ceiling (TLV-TWA) (0.37mg/m3, 0.3 ppm). N-methylenvaline distribution in blood, as measured by GC/MS upon derivatization, showed direct positive relationship to F exposure, with r = 0.340. Prevalence of the molecular adduct expressed in pmol/g of globin was significantly higher in the exposed group (p<0.04) than in the control group. However, the methylenvaline marker was unable to provide significant distinction between the subjects exposed to F through tobacco smoke habit and the non smokers. Despite this interference the usefulness of N-methylenvaline as a biomarker for testing occupational exposure to F was demonstrated.

N-Methylenvaline in a group of subjects occopationally exposed to formaldehyde

BONO, Roberto;VINCENTI, Marco;SCHILIRO', Tiziana;SCURSATONE, Vincenzo;PIGNATA, Cristina;GILLI, Giorgio
2006

Abstract

Aim of this study was to correlate the human exposure to Formaldehyde (F) with N-methylenvaline, a molecular adduct formed by addiction of F to the N-terminal valine in hemoglobin. A group of 21 subjects employed in a plywood factory and a laminate factory, and occupationally exposed to F, together with a group of 30 controls, were recruited as volunteers to test this biomarker. Each subject received a questionnaire and a passive personal F sampler. Exposure to F vapors and occurrence of N-methylenvaline in blood were measured. Integrated F concentrations always proved lower than Threshold Limit Value as a ceiling (TLV-TWA) (0.37mg/m3, 0.3 ppm). N-methylenvaline distribution in blood, as measured by GC/MS upon derivatization, showed direct positive relationship to F exposure, with r = 0.340. Prevalence of the molecular adduct expressed in pmol/g of globin was significantly higher in the exposed group (p<0.04) than in the control group. However, the methylenvaline marker was unable to provide significant distinction between the subjects exposed to F through tobacco smoke habit and the non smokers. Despite this interference the usefulness of N-methylenvaline as a biomarker for testing occupational exposure to F was demonstrated.
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Formaldehyde; n-Methylenvaline; Occupational exposure; Molecular epidemiology
R. BONO; M. VINCENTI; T. SCHILIRO'; E. SCURSATONE; C. PIGNATA; G. GILLI
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/45545
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