Avoiding chemical fixation and solvent dehydration, neutral lipids and phospholipids can be preserved and stained (with fluorochrome Nile Red) in semi-thin section of a glycol methacrylate resin. Moreover, avoiding chemical fixation and solvent dehydration, some enzymes (alkaline phosphatase and delta5-3beta-hydroxy-steroid-dehydrogenase) are better conserved then after formalin fixation and acetone dehydration.
Histochemistry on glycol methacrylate sections, avoiding chemical fixation and dehydration. Enzymes and lipids
DORE, Bruno Emilio;USAI, Pasquale;PATTONO, Paola Maria
1995-01-01
Abstract
Avoiding chemical fixation and solvent dehydration, neutral lipids and phospholipids can be preserved and stained (with fluorochrome Nile Red) in semi-thin section of a glycol methacrylate resin. Moreover, avoiding chemical fixation and solvent dehydration, some enzymes (alkaline phosphatase and delta5-3beta-hydroxy-steroid-dehydrogenase) are better conserved then after formalin fixation and acetone dehydration.
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