Fifteen clones of the cultivar ‘Nebbiolo’, chosen as belonging to 5 biotypes (Lampia, Bolla, Rosé, Michet and Chiavennasca) according to the traditional classification of this cultivar in its growing area in North-West Italy, were examined for morphological and biometrical characters and for DNA profiles using microsatellite markers. The aim of the research was to investigate the phenotypical variability already observed for ‘Nebbiolo’ and to verify the existence of genetic diversity within the cultivar. Morphological observations were carried out using the OIV descriptors for shoot, leaf and fruit. Leaf samples were examined by biometrical techniques. For the DNA typing 5 microsatellite loci were analysed by an Applied Biosystem 373A DNA sequencing apparatus using GENESCAN software and by electrophoresis on sequencing gel, followed by chemiluminescence detection. The morphological and biometrical differences revealed the existence of 3 groups of clones. The first group includes 3 biotypes: Lampia and Bolla, grown in the area of Alba (Piedmont) where Barolo and Barbaresco wines are produced, and Chiavennasca, from Valtellina in Lombardy. The biotypes Rosé and Michet (both from Alba) were distinct from the other ‘Nebbiolo’ biotypes and from each other. The morphologial differences found in the ‘Michet’ clones are mainly due to the presence of the grapevine fanleaf virus. DNA analysis showed that the biotype ‘Rosé’ is genetically distinct from the others, although closely related to them: DNA typing distinguished 2 profiles, one for the ‘Rosé’, and a second shared by all the other genotypes. The results indicate that at least 2 causes of phenotypical polymorphism should be considered for explaining the variability observed in grapevine cultivars of ancient origin: vine phytosanitary conditions and genetic diversity.
Within cultivar grapevine variability studied by morphometrical and molecular marker based techniques
BOTTA, Roberto;AKKAK, Aziz;
2000-01-01
Abstract
Fifteen clones of the cultivar ‘Nebbiolo’, chosen as belonging to 5 biotypes (Lampia, Bolla, Rosé, Michet and Chiavennasca) according to the traditional classification of this cultivar in its growing area in North-West Italy, were examined for morphological and biometrical characters and for DNA profiles using microsatellite markers. The aim of the research was to investigate the phenotypical variability already observed for ‘Nebbiolo’ and to verify the existence of genetic diversity within the cultivar. Morphological observations were carried out using the OIV descriptors for shoot, leaf and fruit. Leaf samples were examined by biometrical techniques. For the DNA typing 5 microsatellite loci were analysed by an Applied Biosystem 373A DNA sequencing apparatus using GENESCAN software and by electrophoresis on sequencing gel, followed by chemiluminescence detection. The morphological and biometrical differences revealed the existence of 3 groups of clones. The first group includes 3 biotypes: Lampia and Bolla, grown in the area of Alba (Piedmont) where Barolo and Barbaresco wines are produced, and Chiavennasca, from Valtellina in Lombardy. The biotypes Rosé and Michet (both from Alba) were distinct from the other ‘Nebbiolo’ biotypes and from each other. The morphologial differences found in the ‘Michet’ clones are mainly due to the presence of the grapevine fanleaf virus. DNA analysis showed that the biotype ‘Rosé’ is genetically distinct from the others, although closely related to them: DNA typing distinguished 2 profiles, one for the ‘Rosé’, and a second shared by all the other genotypes. The results indicate that at least 2 causes of phenotypical polymorphism should be considered for explaining the variability observed in grapevine cultivars of ancient origin: vine phytosanitary conditions and genetic diversity.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
