An electrochemical enzyme-linked immunosorbent assay (ELISA) for the detection of 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane (p,p'-DTT), 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE), 1,1-dichloro-2,2-bis-(4-chlorophenyl)ethane (p,p'- DDD) and o,p-DDT was developed. Optimization of the ELISA competition conditions, led to similar response for the p,p'- isomers. The activity of the label enzyme (horseradish peroxidase) was measured electrochemically using 3,3',5,5'- tetramethylbenzidine as substrate. The use of purified antiserum for p,p'-DDT resulted in a sensitive assay with a detection limit of 40 pg ml−1 and R.S.D. ranging from 1 to 3% intra-day and 3 to 6% inter-day. No matrix effect for waste water samples of different origin has been evidenced. The ELISA was used to detect DDTs in 20 samples after extraction in diethylether. This method appears suitable for routine screening of DDTs without sample pre-treatment other than dilution in PBS or after organic solvent extraction if high sensitivity is required
Electrochemical ELISA for the screening of DDT related compounds: analysis in waste waters
GIRAUDI, Gianfranco;
2003-01-01
Abstract
An electrochemical enzyme-linked immunosorbent assay (ELISA) for the detection of 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane (p,p'-DTT), 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE), 1,1-dichloro-2,2-bis-(4-chlorophenyl)ethane (p,p'- DDD) and o,p-DDT was developed. Optimization of the ELISA competition conditions, led to similar response for the p,p'- isomers. The activity of the label enzyme (horseradish peroxidase) was measured electrochemically using 3,3',5,5'- tetramethylbenzidine as substrate. The use of purified antiserum for p,p'-DDT resulted in a sensitive assay with a detection limit of 40 pg ml−1 and R.S.D. ranging from 1 to 3% intra-day and 3 to 6% inter-day. No matrix effect for waste water samples of different origin has been evidenced. The ELISA was used to detect DDTs in 20 samples after extraction in diethylether. This method appears suitable for routine screening of DDTs without sample pre-treatment other than dilution in PBS or after organic solvent extraction if high sensitivity is required
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