Grapevines transformed to over-express an aquaporin gene were tested under both irrigated and droughted conditions. We measured: gas exchanges by Infra Red Gas Analyzer, root hydraulic conductivity (Khroot) by High Pressure Flow Meter (HPFM), and embolism formation and recovery in petioles by HPFM differential application as influenced by transient flushing pressure. In irrigated conditions, assimilation, stomatal conductance, transpiration and leaf surface were significantly higher in transgenic plants than in wild types. Higher stomatal conductance of transgenic plants was related to a higher Khroot. Transgenic plants embolized upon environmental conditions not causing embolism in wild types, probably in relation to transpiration levels higher in transgenic lines. Overexpression of VvPIP2;4 ameliorated growth performances by modifying water metabolism under no water restrictions, whereas did not induce a drought stress resistance. Transcript level of the endogenous VvPIP2;4 in transgenic lines, analyzed by RT-PCR assay, suggests a molecular compensation mechanism related to ecophysiological behavior.

Changes in ecophysiological performances in grapevines overexpressing the aquaporin VvPIP2;4.

LOVISOLO, Claudio;PERRONE, Irene;SCHUBERT, Andrea
2010

Abstract

Grapevines transformed to over-express an aquaporin gene were tested under both irrigated and droughted conditions. We measured: gas exchanges by Infra Red Gas Analyzer, root hydraulic conductivity (Khroot) by High Pressure Flow Meter (HPFM), and embolism formation and recovery in petioles by HPFM differential application as influenced by transient flushing pressure. In irrigated conditions, assimilation, stomatal conductance, transpiration and leaf surface were significantly higher in transgenic plants than in wild types. Higher stomatal conductance of transgenic plants was related to a higher Khroot. Transgenic plants embolized upon environmental conditions not causing embolism in wild types, probably in relation to transpiration levels higher in transgenic lines. Overexpression of VvPIP2;4 ameliorated growth performances by modifying water metabolism under no water restrictions, whereas did not induce a drought stress resistance. Transcript level of the endogenous VvPIP2;4 in transgenic lines, analyzed by RT-PCR assay, suggests a molecular compensation mechanism related to ecophysiological behavior.
ASPB Plant Biology 2010
Montreal
31.07.10 - 04.08.10
ASPB Proceedings 2010
ASPB
383
384
http://abstracts.aspb.org/pb2010/public/P15/P15031.html
Claudio Lovisolo; Walter Chitarra; Irene Perrone; Giorgio Gambino; Marco Vitali; Ivana Gribaudo; Andrea Schubert
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/74688
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