Pulp of fleshy fruits is extremely rich in water. As a consequence the classical protocols of fixation and embedding in hydrophilic media produce severe shrinkage of the tissue with critical damages to the cell walls. We tested the embedding in hydrophilic glycol-methacrylate resin (Technovit 7100 - Kulzer). The cell walls are less shrinked than with classical protocol, and the results are achieved in a shorter time and with a less expensive procedure.

Fruit pulp semi-thin sections for optical microscopy

JONA, Roberto;DORE, Bruno Emilio;DONNA, Daniela
1998

Abstract

Pulp of fleshy fruits is extremely rich in water. As a consequence the classical protocols of fixation and embedding in hydrophilic media produce severe shrinkage of the tissue with critical damages to the cell walls. We tested the embedding in hydrophilic glycol-methacrylate resin (Technovit 7100 - Kulzer). The cell walls are less shrinked than with classical protocol, and the results are achieved in a shorter time and with a less expensive procedure.
8th International Cell Walls Meeting
Norwich
1-5 settembre 1998
Cell Walls '98
Cell Walls '98
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fleshy fruits; semi-thin sections; optical microscopy; glycol-methacrylate
JONA R.; DORE B.; DONNA D.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/78090
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