Species identification is an essential condition for both basic and applied entomological research as well as for the application of proper control strategies against insect pests. DNA molecular markers offer a valuable support to the traditional morphology-based taxonomy. Mitochondrial DNA sequences and internal transcribed spacer regions (ITS) of the ribosomal operon proved to be effective in insect species differentiation and phylogeny. We developed new molecular taxonomic keys within the family Cixiidae and the tribe Empoascini (family Cicadellidae) based on the mitochondrial cytochrome oxidase I gene (mtCOI) and ITS2 region. PCR-RFLP profiles of both sequences were species-specific within Hyalesthes and Reptalus species (Cixiidae). These identification tools were validated on samples collected in a wide geographic area, from Southern, Central and Eastern Europe. The development of species-specific markers for the Empoascini is ongoing. The molecular keys make the species identification feasible for the non-specialist entomologists and allow the recognition (at all developmental stages) of closely-related and cryptic species. They also contribute to depict the phylogenetic relationships among the species and contribute to the mtCOI-based universal system of animal species identification (“Barcoding life database”).

Molecular markers for insect species identification: case-studies within Hemiptera: Auchenorryncha

BERTIN, SABRINA
2010-01-01

Abstract

Species identification is an essential condition for both basic and applied entomological research as well as for the application of proper control strategies against insect pests. DNA molecular markers offer a valuable support to the traditional morphology-based taxonomy. Mitochondrial DNA sequences and internal transcribed spacer regions (ITS) of the ribosomal operon proved to be effective in insect species differentiation and phylogeny. We developed new molecular taxonomic keys within the family Cixiidae and the tribe Empoascini (family Cicadellidae) based on the mitochondrial cytochrome oxidase I gene (mtCOI) and ITS2 region. PCR-RFLP profiles of both sequences were species-specific within Hyalesthes and Reptalus species (Cixiidae). These identification tools were validated on samples collected in a wide geographic area, from Southern, Central and Eastern Europe. The development of species-specific markers for the Empoascini is ongoing. The molecular keys make the species identification feasible for the non-specialist entomologists and allow the recognition (at all developmental stages) of closely-related and cryptic species. They also contribute to depict the phylogenetic relationships among the species and contribute to the mtCOI-based universal system of animal species identification (“Barcoding life database”).
2010
www.europhd-insectbiotechnology.net
Bertin S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/81515
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