We have developed and validated a high-performance liquid chromatography method coupled with a mass detector to quantify itraconazole, voriconazole, and posaconazole using quinoxaline as the internal standard. The method involves protein precipitation with acetonitrile. Mean accuracy (percent deviation from the true value) and precision (relative standard deviation percentage) were less than 15%. Mean recovery was more than 80% for all drugs quantified. The lower limit of quantification was 0.031 microg/ml for itraconazole and posaconazole and 0.039 microg/ml for voriconazole. The calibration range tested was from 0.031 to 8 microg/ml for itraconazole and posaconazole and from 0.039 to 10 microg/ml for voriconazole.

Development, validation, and routine application of a high-performance liquid chromatography method coupled with a single mass detector for quantification of itraconazole, voriconazole, and posaconazole in human plasma.

BAIETTO, LORENA
First
;
D'AVOLIO, ANTONIO;DE ROSA, Francesco Giuseppe;SICCARDI, MARCO;SIMIELE, MARCO;SCIANDRA, Mauro;DI PERRI, Giovanni
Last
2010-01-01

Abstract

We have developed and validated a high-performance liquid chromatography method coupled with a mass detector to quantify itraconazole, voriconazole, and posaconazole using quinoxaline as the internal standard. The method involves protein precipitation with acetonitrile. Mean accuracy (percent deviation from the true value) and precision (relative standard deviation percentage) were less than 15%. Mean recovery was more than 80% for all drugs quantified. The lower limit of quantification was 0.031 microg/ml for itraconazole and posaconazole and 0.039 microg/ml for voriconazole. The calibration range tested was from 0.031 to 8 microg/ml for itraconazole and posaconazole and from 0.039 to 10 microg/ml for voriconazole.
2010
54
8
3408
3413
http://dx.doi.org/10.1128/AAC.01807-09
L. Baietto;A. D'Avolio;G. Ventimiglia;F. G. De Rosa;M. Siccardi;M. Simiele;M. Sciandra;G. Di Perri
File in questo prodotto:
File Dimensione Formato  
Antimicrob. Agents Chemother.-2010-Baietto-3408-13.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 483.99 kB
Formato Adobe PDF
483.99 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/81771
Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 52
  • ???jsp.display-item.citation.isi??? 46
social impact