A bioactive and fluoroapatite-containing glass-ceramic was used to realize scaffolds (Fa-GC) with the polymeric sponge method. In order to confer antibacterial properties, silver was introduced in the material (Ag-Fa-GC) through a patented ion-exchange process. Scaffolds structure, morphology and composition were characterized through XRD, SEM and EDS analyses; the total porosity, the pore size and the degree of pore interconnection were respectively investigated by means of density measurement, image analysis and capillarity test. The mechanical strength was estimated through compressive test. Released Ag ions were evaluated using the GFAAS analysis and in vitro tests were performed soaking Ag- and Fa-GC in SBF solution. Antibacterial properties were verified through the dilution broth test and the measurement of inhibition zone, using a S. Aureus standard stock. Osteoblasts cultures were realized to investigate material biocompatibility and Ag effect. Fa-GC with an open and interconnected porosity and a compression strength of 2.0 ± 0.6 MPa were successfully obtained. Ag-Fa-GC maintain their bioactivity after the ion-exchange treatment and the introduced Ag was gradually released in SBF. The Ag-Fa-GC showed a good antibacterial behaviour, since they are able to create a significant inhibition halo and limit the bacterial proliferation. Nevertheless the preliminary cytotoxicity test showed that Ag presence affects cells proliferation and viability.

Antibacterial Ag-doped glass-ceramic scaffolds

MAINA, Giovanni;CANUTO, Rosa Angela;SARACINO, Silvia;MUZIO, Giuliana;
2010

Abstract

A bioactive and fluoroapatite-containing glass-ceramic was used to realize scaffolds (Fa-GC) with the polymeric sponge method. In order to confer antibacterial properties, silver was introduced in the material (Ag-Fa-GC) through a patented ion-exchange process. Scaffolds structure, morphology and composition were characterized through XRD, SEM and EDS analyses; the total porosity, the pore size and the degree of pore interconnection were respectively investigated by means of density measurement, image analysis and capillarity test. The mechanical strength was estimated through compressive test. Released Ag ions were evaluated using the GFAAS analysis and in vitro tests were performed soaking Ag- and Fa-GC in SBF solution. Antibacterial properties were verified through the dilution broth test and the measurement of inhibition zone, using a S. Aureus standard stock. Osteoblasts cultures were realized to investigate material biocompatibility and Ag effect. Fa-GC with an open and interconnected porosity and a compression strength of 2.0 ± 0.6 MPa were successfully obtained. Ag-Fa-GC maintain their bioactivity after the ion-exchange treatment and the introduced Ag was gradually released in SBF. The Ag-Fa-GC showed a good antibacterial behaviour, since they are able to create a significant inhibition halo and limit the bacterial proliferation. Nevertheless the preliminary cytotoxicity test showed that Ag presence affects cells proliferation and viability.
8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8
Vancouver
31 maggio - 5 giugno 2009
Advances in Bioceramics and Biotechnologies Ceramic Transactions
John Wiley & Sons
218
77
84
9780470905487
Antibacterial properties; Compression strength; Compressive tests; Cytotoxicity test; Density Measurements; Doped glass; Fluoroapatite; In-vitro tests; Inhibition zones; Interconnected porosity; Ion exchange process; Ion-exchange treatment; Mechanical strength; Morphology and composition; Polymeric sponge method; S. aureus; SBF solution; SEM and EDS analysis; Total porosity; XRD Engineering controlled terms: Bioceramics; Biocompatibility; Cell culture; Compressive strength; Image analysis; Ion exchange; Ion exchangers; Ions; Polymeric glass; Scaffolds; Secondary batteries; Testing Engineering main heading: Bioactive glass
Vernè E; Miola M; Balagna C; Fucale G; Maina G; Canuto RA; Saracino S; Muzio G; Vitale-Brovarone C.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/82207
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