Tributyltin (TBT) is a largely diffused environmental pollutant, banned from paints in the European Union from 2003. However, the level of TBT (and other organotins) in food, particularly fish and shellfish, remains still high. Several studies demonstrated that TBT is involved in the development of obesity, via peripheral action, but currently, there are only a few data illustrating effects of TBT on the nervous system. In the present study, we tested the hypothesis that acute exposure to TBT may directly activate brain cells in particular, in those hypothalamic nuclei regulating the food intake. To this purpose, TBT was orally administered at a single dose (10mg/kg/body weight) to two groups of adult male mice: regularly fed or fasted for 24h. Mice were sacrificed 90min after the TBT administration and perfused by 4% paraformaldehyde. Brains were quickly dissected, frozen and sectioned for immunocytochemical detection of c-fos, a common marker of cell activation. In both, fed or fasted mice, exposure to TBT induced a significant increase of c-fos expression in the arcuate nucleus in comparison to control mice. The other nuclei involved in the control of feeding behavior did not show any significant increase. These data are the first in vivo demonstration that TBT has not only peripheral effects, but also may activate elements in the brain, in particular in a crucial region for the regulation of food intake like the arcuate nucleus.

ACUTE EXPOSURE TO TRIBUTYLTIN INDUCES C-FOS ACTIVATION IN THE HYPOTHALAMIC ARCUATE NUCLEUS OF ADULT MALE MICE

BO, Elisabetta;VIGLIETTI, Carla Maria;PANZICA, Giancarlo
2011

Abstract

Tributyltin (TBT) is a largely diffused environmental pollutant, banned from paints in the European Union from 2003. However, the level of TBT (and other organotins) in food, particularly fish and shellfish, remains still high. Several studies demonstrated that TBT is involved in the development of obesity, via peripheral action, but currently, there are only a few data illustrating effects of TBT on the nervous system. In the present study, we tested the hypothesis that acute exposure to TBT may directly activate brain cells in particular, in those hypothalamic nuclei regulating the food intake. To this purpose, TBT was orally administered at a single dose (10mg/kg/body weight) to two groups of adult male mice: regularly fed or fasted for 24h. Mice were sacrificed 90min after the TBT administration and perfused by 4% paraformaldehyde. Brains were quickly dissected, frozen and sectioned for immunocytochemical detection of c-fos, a common marker of cell activation. In both, fed or fasted mice, exposure to TBT induced a significant increase of c-fos expression in the arcuate nucleus in comparison to control mice. The other nuclei involved in the control of feeding behavior did not show any significant increase. These data are the first in vivo demonstration that TBT has not only peripheral effects, but also may activate elements in the brain, in particular in a crucial region for the regulation of food intake like the arcuate nucleus.
32
277
280
Tributyltin; Endocrine disruptors; food intakes; Hypothalamus
Elisabetta Bo; Carla Maria Viglietti; Giancarlo Panzica
File in questo prodotto:
File Dimensione Formato  
2011BoNeurotox.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 435.95 kB
Formato Adobe PDF
435.95 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
NEUTOX-S-10-00265.pdf.pdf

Accesso riservato

Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 286.91 kB
Formato Adobe PDF
286.91 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/87475
Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 23
  • ???jsp.display-item.citation.isi??? 21
social impact