Molecular genetic methods have several advantages over classical morphological and chemical analyses. The genetic method requires genotype instead than phenotype, therefore PCR-based techniques have been widely used for a rapid identifi cation of plant species, varieties and chemotypes. Recently, the molecular discrimination of some higher plant species has been evaluated using sequences of a 5S-rRNA gene spacer region. The variation in the nontranscribed sequence (NTS) region has been used in a number of plant species for studying intraspecifi c variation, genome evolution, and phylogenetic reconstruction. Here, we describe a rapid method based on the use of the 5S-rRNA-NTS region as a tool for plant DNA fi ngerprinting, which combines PCR, sequencing and restriction fragment length polymorphism analyses.

Restriction Fragment Length Polymorphism (RFLP) of the 5S-rRNA-NTS region: a rapid and precise method for plant identification

BERTEA, CINZIA MARGHERITA;GNAVI, Giorgio
2012-01-01

Abstract

Molecular genetic methods have several advantages over classical morphological and chemical analyses. The genetic method requires genotype instead than phenotype, therefore PCR-based techniques have been widely used for a rapid identifi cation of plant species, varieties and chemotypes. Recently, the molecular discrimination of some higher plant species has been evaluated using sequences of a 5S-rRNA gene spacer region. The variation in the nontranscribed sequence (NTS) region has been used in a number of plant species for studying intraspecifi c variation, genome evolution, and phylogenetic reconstruction. Here, we describe a rapid method based on the use of the 5S-rRNA-NTS region as a tool for plant DNA fi ngerprinting, which combines PCR, sequencing and restriction fragment length polymorphism analyses.
2012
Plant DNA Fingerprinting and Barcoding: Methods and Protocols
Humana Press
89
101
9781617796081
http://dx.doi.org/10.1007/978-1-61779-609-8_8
DNA fi ngerprinting , 5S-rRNA gene , Nontranscribed sequence region , PCR–RFLP , High-resolution gel capillary electrophoresis , Agilent 2100 Bioanalyzer
C.M. Bertea; G. Gnavi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/91130
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