The main event associated with the interaction of nanoparticles with biological fluids is the formation of a protein corona. Predicting the fate of NPs in biological environments implies a thorough characterization of such protein layer in terms of chemical composition of the NP surface as well as structural characterization. Both the changes in protein fold and the selective orientation of proteins onto NPs may influence the final outcome of NP in the body. In this paper we provide some examples of how EPR spectroscopy coupled to site-directed spin-labeling (SDSL) may shed light on these specific aspects related to the protein adsorption mechanisms.

SDSL-EPR as a tool for grasping topological information on the interaction between proteins and nanoparticles

GHIBAUDI, Elena Maria;BOSCOLO, BARBARA;TURCI, Francesco;FENOGLIO, Ivana;FUBINI, Bice
2011-01-01

Abstract

The main event associated with the interaction of nanoparticles with biological fluids is the formation of a protein corona. Predicting the fate of NPs in biological environments implies a thorough characterization of such protein layer in terms of chemical composition of the NP surface as well as structural characterization. Both the changes in protein fold and the selective orientation of proteins onto NPs may influence the final outcome of NP in the body. In this paper we provide some examples of how EPR spectroscopy coupled to site-directed spin-labeling (SDSL) may shed light on these specific aspects related to the protein adsorption mechanisms.
4th European Conference on Chemistry for Life Sciences
Budapest
2011
Proceedings of the 4th European Conference on Chemistry for Life Sciences
Medimont
25
28
9788875876319
Nanoparticles; EPR spectroscopy; proteins
Ghibaudi E.M.; Boscolo B.; Turci F.; Fenoglio I.; Fubini B.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/92340
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