Production of H2O2 was stimulated in cell-free crude homogenates from callus of melon, treated with hyphal wall components of Fusarium oxysporum f.sp. melonis. Active oxygen production was detected as PO-catalized oxidative bleaching of a fluorescent probe (pyranine) occurred when the homogenate of a resistant melon genotype was treated with HWC of an avirulent race of Fusarium, but not when a non resistant plant genotype or a virulent race of the fungus were included in the incubation mixture. The role of a NADPH-dependent membrane oxidase and the role of a LOX/fatty acid system were discussed.

Production of active oxygen species elicited in homogenates of Cucumis melo by hyphal wall components of Fusarium oxysporum f.sp. melonis:involvement of the lipoxygenase/fatty acid system

DE DONATO, Mariano;MOZZETTI MONTERUMICI, Chiara;CHIAVAZZA, Paola Maria;MATTA, Alberto
1996-01-01

Abstract

Production of H2O2 was stimulated in cell-free crude homogenates from callus of melon, treated with hyphal wall components of Fusarium oxysporum f.sp. melonis. Active oxygen production was detected as PO-catalized oxidative bleaching of a fluorescent probe (pyranine) occurred when the homogenate of a resistant melon genotype was treated with HWC of an avirulent race of Fusarium, but not when a non resistant plant genotype or a virulent race of the fungus were included in the incubation mixture. The role of a NADPH-dependent membrane oxidase and the role of a LOX/fatty acid system were discussed.
1996
Physiological and molecular aspects of the plant-pathogen interactions
ragusa Ibla
31 maggio - 1 giugno
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229
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Fusarium oxysporum f.sp. melonis; active oxygen production; pyranine
De Donato M.; Mozzetti C.; Chiavazza P.; Rubino R.; Matta A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/128422
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